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A COMPREHENSIVE REVIEW OF VARIOUS LASER-BASED SYSTEMS USED IN EARLY DETECTION OF DENTAL CARIES Figure 4. Multispectral near-infrared reflectance imaging: (a) clinical presentation of caries on the tooth; (b) cross-section of tooth with caries; (c) Multispectral near-infrared reflectance imaging (Source of image: Zakian C et al. Nearinfrared hyperspectral imaging of teeth for dental caries detection. J Biomed Opt. 2009;14(6):064047) and compared that range of contrast with visible reflectance and LF. Forty-four human molars were painted with an acid-resistant varnish leaving a window (4 mm×4 mm) on the occlusal surface of each tooth exposed for demineralization. In the unprotected windows, artificial lesions were induced using a demineralizing solution at pH 4.5. Near-IR reflectance images were obtained over several near-IR spectral distributions, visible light reflectance and LF with 405 nm excitation and detection at wavelengths greater than 500 nm. In each sample, the severity of the demineralization window was non-invasively assessed using cross polarization optical coherence tomography (CPOCT). The CP-OCT results showed that the severity of the carious lesions varied significantly across the sample windows and that visible light does not precisely reflect the large variation in the lesion severity as compared to near-IR reflectance. Reflectance measurements at certain nearIR wavelengths have been reported to more accurately reflect variation in the depth and severity of the lesions. 27 Chung et al. 27 reported that near-IR measurements at higher wavelengths (1,200nm to 1,600nm) reflect the variation in the depth and severity of the lesions more accurately as compared to shorter wavelengths. In another study, Wu and Fried28 compared the image contrast of artificial carious lesions produced on the buccal and occlusal surfaces of teeth using fluorescence, visible reflectance and near-IR reflectance. The results demonstrated that near-IR reflectance at wavelength 1,310-nm yielded the greatest contrast than visible reflectance though it remained statistically comparable with fluorescence imaging.28 In a recent study Söchtig et al.29 suggested that NIR transillumination is a method that may help avoid bitewing radiographs for diagnosis of caries in routine dental practice. However, further studies over a greater range of lesion severity are needed to determine the optimum performance ranges for this imaging technique. Conclusion Lasers-based caries systems are useful tools in the early detection of caries as compared to traditional visual and radiographic examination techniques; however, further studies are needed to assess the caries diagnostic efficacy of the various commercially available laser-based caries diagnostic systems. Conflict of interest and financial disclosure The authors declare that they have no conflict of interest and there was no external source of funding for the present study. 111