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DENTAL CARIES Figure 2. Light emitting diode caries detection system (Source of image: Rodrigues JA et al. Light-emitting diode and laser fluorescence–based devices in detecting occlusal caries. J Biomed Opt. 2011;16(10):107003) Figure 3. Fluorescence camera based caries detection system (Source of image: http://www.airtechniques.com/ products/cam-x-spectra-caries-detection-aid.html) caries following which they were assessed with the LED- and LF-based caries detection devices13. The study concluded that LED-based and LFbased devices detect caries more accurately as compared to traditional visual-examination based methodologies.13 However, the result showed no significant difference in the caries detecting efficacy of LEDand LF-based systems.13 Results by Rodriguez et al.14 demonstrated that LED-based caries diagnosis systems are unable to differentiate sound surfaces from enamel caries. In this study14, the laser parameters used in the LED-based caries detection system remained unclear. In this regard, further studies with a large sample size are needed to assess the significance of LED-based systems in the diagnosis of caries. Fluorescence cameras Achilleos et al. 20, introduced a fluorescence device (FC; Vista Proof, Duerr Dental, Germany), to detect caries on occlusal surfaces of teeth ( Fig.3). The tool emits light with a wavelength of 400 nm and filters the fluorescence emitted by the tissue. 110 Specific software quantifies the fluorescence on a numerical scale ranging from 0 to 5. This device also works on the same principle as that for DIAGNOdent that is, capturing the fluorescence from bacterial porphyrins. The authors reported the sensitivity of Vista Proof to be significantly greater that for DIAGNOdent (pen).20 In another study, Seremidi et al.21 compared the efficacy of fluorescence device VistaProof (VP) and DIAGNOdent Pen. In this study, 107 sites on 41 occlusal surfaces of recently extracted premolars were selected and classified into lesion categories according to Ekstrand’s clinical criteria [21]. The fluorescence of the sites was measured by the Vista Proof and DIAGNOdent Pen devices. The results demonstrated no statistically significant difference between the accuracy of DP and VPs for both enamel and dentin lesions. 21 Multispectral near-infrared reflectance imaging  Near infrared (NIR) multispectral imaging is a novel non-invasive imaging technology that maps and quantifies caries without using ionizing radiation.22-25 This technology works on the concept that for longer wavelengths (such as 1450 nm), water absorption increases significantly and reduces the penetration of the NIR light (Fig.4). 26 NIR digital imaging transillumination technology has been shown to be useful as a diagnostic tool in the detection of proximal caries and, less importantly, for occlusal caries, fissures, and secondary decay around amalgam and composite restorations. 25 It has also been reported that early demineralization appears with high contrast at NIR wavelengths due to a 10- to 20-fold difference in the magnitude of light scattering between sound and demineralized enamel. 22,26 In an in-vitro study, Simon et al. 26 assessed how the lesion contrast changes with the lesion severity and depth for different spectral regions in H