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Figure 8. Third patient: on the left, the 3D model from the first cast, showing a malposition of the left canine; on the right, the 3D model from the second cast, after orthodontic therapy. Interestingly, RMS value increased with the entity of dental displacement and number of involved teeth, and was in every case significantly higher than the same parameter shown by the control group (Table 1). Figure 9. Third patient, chromatic map of modifications of dental surfaces between the two casts: blue areas are more prominent in the last cast, vice versa for the red and yellow areas. Most of modifications can be observed on the left central incisor and the left canine. but for the left canine which remained in the same position and was realigned within the dental arch (Fig. 7). In addition, the method was able to accurate describe the novel presence of brackets and wire in the second cast, correctly assessed in blue areas (more vestibularized in the second cast than in the first one). The average RMS value was 1.13 mm. The third patient was an 11 year old girl, who was chosen in order to test the detectability of lesser dental displacements like pathological overjet value. In detail, the central incisors were distally rotated, whereas the right canine was still erupting with the exposition of the tip. In the second cast, after orthodontic therapy, the central incisors were medially oriented, whereas the canine crown was erupted and in correct occlusion. The second cast was taken after four years. The registration procedure correctly assessed the change in orientation of the left central incisor: in addition, the eruption of the canine was detected as well (Fig. 9); RMS value was 0.98 mm. Stomatology Edu Journal QUANTIFICATION OF DENTAL MOVEMENTS IN ORTHODONTIC FOLLOW-UP: A NOVEL APPROACH BASED ON REGISTRATION OF 3D MODELS OF DENTAL CASTS 4. Discussion In the last century orthodontics has seen a progressive update of technologies and clinical procedures, with an amelioration of dental position, functionality and aesthetics. 1 On the other side, a parallel issue concerns the assessment of dental displacement powered by orthodontic therapy, in order to verify the clinical success and provide corrections. Surprisingly, although the constant development of 3D image acquisition systems has represented a crucial revolution in dentistry, their application to the field of orthodontics is still at the beginning and most of their potentiality remains to be explored. 8 An example is provided by Thirvenkatachari et al. who proposed a protocol for the registration of 3D surfaces and calculation of displacement of the center of mass of dental elements. 8 This type of approach provides a metrical information but is not able to predict the modifications of the entire dental surfaces, especially where the movements do not consider dental translation. Another important aspect concerns the morphological assessment of dental movements, which may give an additional information for the evaluation of orthodontic therapies. The present protocol may represent a proposal for an innovative analysis of dental movements: registration is based on the morphology of palatal rugae which are stable with time 12,13 and have been already used as reference point in 3D-3D superimposition of dental arches. 14 The procedure is repeatable and provides both morphological and metrical analyses of dental movements. The chromatic map of dental arches can give information concerning the specific movement of each dental element (rotation, translation or inclination), immediately readable by the operator. 59