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Figure 4. First patient: on the left, the 3D model from the first cast, showing a malposition of both canines; on the right, the 3D model from the second cast, after one year and the removal of the second premolar on the right side and the first on the left side, and consequent realignment of canines. QUANTIFICATION OF DENTAL MOVEMENTS IN ORTHODONTIC FOLLOW-UP: A NOVEL APPROACH BASED ON REGISTRATION OF 3D MODELS OF DENTAL CASTS Figure 5. first patient, chromatic map of modifications of dental surfaces from the left side between the two casts (on the left vestibular surface, on the right lingual surface): blue areas are more vestibularized in the last cast, vice versa for the red and yellow areas. Green areas (including the first and second molar) remained unchanged. an orthodontic treatment in a private dental office: all the patients were IOTN (index of orthodontics treatment needs) ≤3. 11 At least two dental casts were available for each patient, taken at different time periods during the therapy. The casts were scanned by a 3D laser scanner (iSeries, Dental Wings©, Montreal, Canada). According to the manufacter, the precision of the instrument is 15 μm. The 3D models were then elaborated through VAM© software (Canfield Scientific, Inc., Fairfield, NJ): first the palatal area including palatal rugae was manually selected in both surfaces (Fig. 1); then the software was requested to automatically register the two models in order to reach the minimum point-to-point distance between the selected areas (Fig. 2). Once the registration between the two surfaces was reached, the dental arch (dental crown surfaces) was manually defined on the 3D model obtained from the more recent cast, and a Region of Interest (RoI) was obtained. The software was then requested to select the RoI and to calculate the point-to point mean distance and RMS value (Root Mean Square) of the two models within the selected Stomatology Edu Journal RoI. Mean values consider together positive and negative movements, whereas RMS values are all positive, and can provide a complete evaluation of the variations between two dental scans. Together with these quantitative parameters, a chromatic map of surface modifications of dental element extracted from the more recent dental cast is provided, with areas coloured in blue, green and red: the blue areas are more vestibularized in the last cast than in the earlier one, whereas the red areas are less vestibularized. Green areas do not show modifications between the two casts (Fig. 3). To test the method on a control group, the same procedure was applied on the dental arch models of five adult patients aged over 18 years who had longitudinal records taken but where no dental movements or modifications were expected. Time elapsed between the two casts was 1.5 years on average. The same procedures of registration, RoI selection, and calculation of RMS values on the control group was repeated by the same operator and by another observer: intra- and inter-observer differences were statistically assessed by Student’s 57