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CARIES DETECTION WITH LASER FLUORESCENCE DEVICES. LIMITATIONS OF THEIR USE The sensitivity of DD can be increased for more deep caries, with values of 0.66 in D2 and 1 in D3. Sensitivity for the D1 level was reported at 0.74. 39 3.2. DIAGNOdent Pen Device The inability of DD to detect approximal caries was the primary cause of creating the DDPen (Fig.1). Figure 1. The handpiece of the DDPen. The DDPen follows the basic principles of the DD model. The main difference is the design of its tip which can be rotated to the longitudinal axis and thus permitting the detection of approximal caries. Also, DDPen uses the same optical sapphire fiber for the distribution of radiation and the detection of tooth fluorescence without the interference of other optical fibers (Fig. 2). Figure 2. The DDPen tip over an occlusal surface of a molar. Two different tips are available; a cylindrical one (CYL) with a diameter of 1.1 mm and a conical one (CON) with a diameter of 0.7mm. Although, the diameter of the CON is about 0.3mm thinner than that of DD and thus it would be expected to show better accuracy on pits and fissures, it seems that there is no significant difference between them 40 (Fig. 3). Figure 3. The different tips of the DDPen. 50 3.2.1. Accuracy and repeatability of DDPen Lussi et al. 40 compared in vitro the accuracy of caries detection by DD and DDPen. In their study, o 119 third molars, kept in frozen state at -20 C, were examined. DDpen showed higher specificity (0.71 to 0.91) compared to the DD (0.69-0.79), but relatively lower sensitivity (0.78 to 0.91) against the latter (0.81 to 0.96). The main limitation of the study is that only third of the molars were used, whose occlusal surface varies considerably in different individuals as compared to other posterior teeth. Kuhnisch et al. 41 found that the reproducibility of DD of the same examiner (0.89) was similar to that of DDPen (0.88), while between different examiners reliability (0.86) was noted. Sinanoglou et al. 42 evaluated in vivo the occlusal surfaces of 217 permanent molars and premolars, comparing the visual observation (ICDASII), DDPen and bitewing radiography. One week after the first measurements, the patients were invited for re-examination and 82 teeth were reassessed with the above-mentioned techniques. Only the teeth with dentine caries were examined (64 of 227) and the clinical depth of the lesion was measured. The reliability of DDPen was moderate to good, with AUC 0.55-0.64, but noticeably inferior in contrast to that of visual observation (AUC 0.71- 0.76) that reached higher specificity values than sensitivity. At this point, it should be mentioned that the results of the evaluation for visual observation could have been affected by the subjective skills and the level of the examiner’s acquaintance with the device. 15 Moreover, the device detection capability was better for dentin caries (D3), a finding supported by many other studies. 14,15,43 The reproducibility for DDPen between different examiners (0.61, 0.65) and the same one (0.59. 0.65) was relatively low (16.42). It is worth noting that in the study by Seremidi et al. 17 the teeth were stored in tap water for a long time, which is likely to have an impact on the fluorescence levels of the teeth. The study by Achilleos et al. 43 revealed low sensitivity values (0.66-0.75) for DDPen, which may be attributed to the fact that the study was focused on the D1 level, where the performance of this device is reduced compared to the D3 level. Additionally, the relatively small number of samples 38 and the only one week period among the two measurements were reported as limitations of this study. Mortensen et al., 44 focusing on the level of D3, showed high repeatability for DDPen between different examiners (0.98). For COV=40, there was a very high specificity (0.97) but very low sensitivity (0.07). The authors support the idea that if the manufacturer’s COV are applied in clinical practice, there will be a significant reduction of overtreatment, but also the detection of caries in D3 will be very low. Stoma Edu J. 2017;4(1): 46-53. http://www.stomaeduj.com