CHITOSAN MODIFIED POLY(LACTIC-CO-GLYCOLIC) ACID NANOPARTICLES INTERACTION WITH
NORMAL, PRECANCEROUS KERATINOCYTES AND DENTAL PULP CELLS
Maria Justina Roxana VIRLAN
DDS, MSc, PhD Student
Department of Biochemistry
Faculty of Dental Medicine, U.M.F.”Carol Davila “Bucharest, Romania
CV
Dr Justina Virlan graduated from the Faculty of Dental Medicine in 2012 and completed an Endodontics
residency. In 2015 she finished a master programme at the Faculty of Medical Engineering. A laureate of the
National Chemistry Competition and a PhD student at the Biochemistry Department of the Dental Medicine
Faculty, she has a high interest in nanomaterials and their applications in dentistry.
Questions
Chitosan modified poly(lactic-co-glycolic) acid nanoparticles (PLGAChi NPs) could be used to
deliver drugs:
q
q
q
q
a.
b.
c.
d.
to the dental pulp cells;
to the oral mucosa;
to the dental pulp cells and oral mucosa;
none of the above.
The PLGAChi NPs (used in this study) can enter :
q
q
q
q
a.
b.
c.
d.
normal oral keratinocytes (NOKs);
precancerous oral keratinocytes (POE9i);
dental pulp cells (DPC);
normal oral keratinocytes (NOKs) and precancerous oral keratinoctes (POE9i).
The multilayered epihelia of oral mucosa was grown in vitro using:
q
q
q
q
a.
b.
c.
d.
collagen matrix;
collagen matrix; normal oral fibroblasts (NOFs);
collagen matrix; normal oral fibroblasts (NOFs); normal oral keratinocytes (NOKs);
collagen and matrigel matrix; normal oral fibroblasts (NOFs); normal oral keratinocytes (NOKs).
In the cell lines that have internalized PLGAChi NPs, the maximum uptake of NPs was observed
after exposure to:
q a. 200 g/mL PLGAChi NPs for 24 h;
q b. 200 g/mL PLGAChi NPs for 12 h;
q c. 20 g/mL PLGAChi NPs for 12 h;
q d. 20 g/mL PLGAChi NPs for 24 h.
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Stoma Edu J. 2017;4(1): 16-26. http://www.stomaeduj.com