Figure 6. Percentage of cells which have internalized PLGAChi NPs from the total amount of exposed cells. NOKs
and POE9i cells ( average values ).
Figure 7. Penetration of PLGAChi NPs in the 3D
organotypic models of human mucosa. The green
fluorescent dots represent the PLGAChi NPs which
were able to enter the reconstituted OT of normal
human mucosa. The sample was exposed to 200µg/mL
PLGAChi NPs for 24h.
In the NOKs and POE9i cell there was no statistical
differenc e between the control and treated cells
(Fig. 8).
4. Discussion
Polymeric NPs are still viewed as the first option for
drug delivery and also widely used in the research
of other diseases. 1-3 Recently, a wide variety of
studies has been undertaken leading the way for
possible future applications of PLGA NPs in a high
number of dental fields, from periodontology and
endodontics to tissue regeneration of skin, bone
or cartilage. 28 Biocompatibility, biodegradability,
flexibility, and minimal side effects are the main
advantages when using PLGA for biomedical
applications. 4 However, the overall negative
charge of these NPs has been reported to diminish
their interaction with the negatively charged
cell membrane, while the rapid opsonization
of hydrophobic PLGA NPs is a major limitation
CHITOSAN MODIFIED POLY(LACTIC-CO-GLYCOLIC) ACID NANOPARTICLES INTERACTION WITH
NORMAL, PRECANCEROUS KERATINOCYTES AND DENTAL PULP CELLS
Figure 8. Viability of NOKs and POE9i after exposure to PLGAChi NPs.
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