Review/Oorsig Volume 22, Issue 04 - Page 28

Oorsig/Review like fungi. Flotation and special staining is best. Immunofluoroscopy probably best. Size 6 micrometer - as far as I remember RBC are 7 micron?? WG - 15 Jan 2017 I find that the test strips usually show positive for Crypto - it is often present in calves without signs so may or may not have been the cause/ only cause in this case. It is also probably present on most/all farms. RL - 26 May 2018 To answer your questions I have noted the following: Cryptosporidium is easily missed if only a single section of small intestine is submitted (organisms are most concentrated in the distal jejunum and ileum). • • • • RL - 27 June 2018 Be very careful about taking action on a Crypto test strip without having confirmatory pathology i.e. histological evidence of severe villous atrophy with villous epithelial colonization by Cryptosporidium and moderate proprial inflammatory infiltrates. Crypto is commonly associated with other extremely virulent enteric pathogens and you don’t want to be missing those. If the farmer really wants to waste money - take action on a screening strip test without any supporting clinical or laboratory data. My 5 cents worth HYGIENE & IMMUNE STATUS MvdL - 14 Feb 2014 Poor fixation procedures of intestine for histopathology is probably the greatest cause of “missed diagnosis”. Ensure you keep your intestine tubes intact, maximum of 2cm in length and gently wash out the ingesta from the lumen with 10% buffered formalin before sinking in your formalin pot for fixation. If you do not flush out the ingesta with formalin and simply place the intestine segment directly into formalin the formalin takes time to penetrate through the ingesta and by then you have post mortal autodigestive change of the superficial villous mucosa and that’s exactly where the crypto sit and they are therefore destroyed. Placing long sections of intestine (6cm plus) directly into formalin is a BIG no no. In an ideal world these calves would have received adequate colostrum. The big dairy beef operators who raise thousands of veal calves work from the assumption that the bull calves have received little or no colostrum & they do not test for TSP. Where did the colostrum go? To the heifer calves. They make it work by raising them in individual hutches, meticulous ѕѥѼхɥ)ɕѼ̰ѥձɱѡ)եиѡ䁡ٔ܁͕ɕ)ѡݽeЁ͡ɔѽQ͔)ՅєЁ͕͕ȁ)!Ё݅ѕȀѕɝЁɔ͕ѥѼɕٔ)ѡЁȁՍ̰ѱ̸̀eԁɔ)剔ЁݥѠɝ՝յȁ)ٕ̰Ё́ɔٕ䁕ѥٕ݅͡)չ䁵Q䁍ѡ)ͥхЁѥЁ͔) ɔԁЁѡ͔)مՑѽ́م̤)ɔԁٔѡ)ͽєمѥ́䁽)ݡɔԁɔݥѠѡ)Ʌ̸ Ѽɽ̸Q䁅ɔՍ)ɔɕͥхЁѼɕѵЁѡLمɥ)ɕձ́ɔхݥѠɥYᅸ)Q͕͔́Յ͕ѥЁ)͔ٽȁݡ%Ёݥ٥ͅѼ)ЁͥɕЁѡٔѡٕ)ѼɕЁɕɥٕՔٕ)ѕɅ䤁݅䁙ɽձЁ܁Ʌѡ)Ё́ɕ͕ٽ̤ͥЁѡɥ)ɅͥɕݥѠɵ䄤) ɕհͽє)́ɥ䁍͔չ́ԁٔ)ѡѡͽє5)ɸЁѼ̽ͅх)ɕɥѡѡ Ѽ)!͕ٔɽѕٕ(ٕ䁡х䤁ɽɕMєЁѡ)݅́䁱ЁݕLѡ́ɔхѥ)Ѽյݸɔ-i8ͼݥ)щɕ́ѡȁѕѡЁѽ)хЁɔ()QĹ))]ԁ)) ɽ Ѽɕ́坥մ)ݡ͕́ѥѡ́ͅȁн)͕́ɡIձȁѽɥ%)ȁQM@ݥѕԁѡմɽɅ