Review/Oorsig Volume 22, Issue 02 - Page 25

Blood Transfusion in Bovines Volume 22 • Issue 02 • 2018 Dr Francois van Niekerk Humansdorp Veterinary Clinic DONOR RECIPIENT Requirements Requirements 1. 2. 3. 4. 5. Xylazine 1.2 ml IM Lignocaine 23g needle Nº 10 or 15 scalpel blade and handle Blood collection needle [4 – 5mm internal diameter, available from Instavet] or trocar & cannula of similar diameter. Large plastic funnel plus plastic tube [latter optional] 6. Container for blood [5 litre anthelmintic container ideal] 7. Anti-coagulant • Na citrate 3.8% solution [add 3.8g to 100ml saline, need 100ml of this solution per litre of blood, thus 19g Na citrate/5litres of blood] • Therefore dissolve 20g Na citrate in 500ml saline per 5ℓ blood 8. Tourniquet for caudal neck 1. High capacity giving set 10 drops/ml and/or flutter valve 2. 12g 8cm IV catheter for administering blood [available from Lakato] 3. Lignocaine [optional] 4. Nº 15 blade [optional] 5. Adrenalin [for transfusion reactions, optional] Procedure (Donor) • 1. Raise head of donor with nose tongs and bend head slightly to side opposite to that on which you will work. 2. Coat funnel and tube with anticoagulant 3. Lignocaine over jugular vein 4. 1.5cm incision over vein 5. More lignocaine in s/c tissue over vein 6. Dissect down onto donor’s jugular [with care!] 7. Grab vein with rat-tooth forceps and hold firmly 8. Stab in collection needle/trocar Note 3.8g Na citrate crystals = a slightly rounded teaspoon Notes • • Extend length of breather pipe on flutter valve to avoid blood running out. Ensure flutter valve not perished, can tear on wider spouts of 5ℓ containers. Large bore blood giving set can be attached to flutter valve. Amount of Blood Required (Guideline) One litre of blood will raise PCV about 0.75%, therefore to raise PCV from 10% to 15% need 5% ÷ 0.75% = 6.66 litres in a 600kg cow. Clinical difference between a PCV of 10% and one of 15% is significant. 25