Corey Goyeneche
Research Teaching
Specialist IV
Department of Neurology
& Neurosciences
UMDNJ, New Jersey
Medical School
NJMS Cancer Center
205 South Orange Ave
Newark, NJ 07103
Dr. Teresa Wood
Professor and Rena
Warshow Chair in Multiple
Sclerosis
Department of Neurology
& Neurosciences
NJMS UH Cancer Center
H1200
205 South Orange
Avenue
Newark, NJ 07103
Ira Augenzucker
Product Line Manager
Labnet International
31 Mayfield Ave
Edison, NJ 08837
Katherine Strathearn
Ph.D.
Cell Application Scientist
Corning Incorporated Life Sciences
2 Alfred Road
Kennebunk, ME 04043
Common laboratory research practice
utilizes PCR to validate transgenic mouse
lines. Validation of these lines typically
involve multiple primer sets with various
annealing temperatures leading to a very
tedious and time consuming process.
To allow researchers the opportunity to
evaluate multiple transgenes within one
PCR reaction, Labnet offers the MultiGene
OptiMax. Traditional thermal cyclers utilize
a Peltier microchip block that is enabled for
either homogeneous or gradient
temperature mode. Additionally, with a
traditional thermal cycler, a user can only
utilize one annealing temperature per
experiment. The new Labnet MultiGene
OptiMax has six distinct Peltier microchip
elements that allow users to select up to six
different annealing temperatures. This
allows for the possibility to evaluate multiple
genes in one experiment.
The focus of this study was to evaluate
the ability of the Labnet MultiGene
OptiMax to perform multiple PCR
reactions with various annealing
temperatures within one experiment. The
mouse line used in this application note is
a transgenic PTD mouse. Typically, the
validation for this line requires three
separate PCR runs that are more than
three hours each. The Labnet MultiGene
OptiMax decreased the time required for
these PCR experiments by completing the
validation with one PCR run, which was
performed in approximately three hours.
The results depicted here demonstrate the
ability to achieve similar product yields
using the Labnet Multigene Optimax
compared to a traditional thermal cycler.
Taken together, the Labnet thermal cycler
provides users with an option for a time
saving programming mode to quickly
validate transgenic mouse lines.