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protein synthesis resulting from more protein being shuttled into muscle tissues via increased blood flow). Arginine For the Win Contrary to what their name may suggest, NO supplements do not contain any actual NO – in fact, the central ingredient in all NO products is the amino acid arginine (or L-arginine), and more recently citrulline malate, a more efficient source of Arginine (nitric oxide itself is scientifically referred to as arginine-alpha-keto-glutarate, or AAKG). To manufacture NO, the body breaks down arginine to set in motion the chain of events outlined above. Though no clear dosing guidelines have been established, it is important to assess your tolerance levels to determine the right NO supplement dosage for your individual needs. Follow label instructions and, if in doubt, consult your physician to determine the safest dosage for you. Additional NO Benefits NO may reduce the possibility of cardiovascular disease in those who exercise regularly. Whenever we work for an extended period, the endothelium of our cells releases NO so it may exert its vasodilation effects to improve blood flow. A major precipitating factor in the formation of atherosclerosis (a plugging, or occlusion of our arteries) is damage to the endothelium. Whenever NO is released it prevents red blood cells from clumping together (or aggregating) and attaching to the endothelium wall, thereby potentially minimizing the possibility of heart disease or stroke. As mentioned, vascular tone is also controlled through NO release; this also serves to keep the arteries clean and clear and our risk of heart disease down. Improved recovery between hard workouts is a further benefit of increased NO levels. Nutrient uptake into damaged muscles has been shown to facilitate the healing process and NO speeds the rate at which nutrients pass through the blood, en route to providing valuable sustenance to sore, depleted muscle fibers. This increased blood flow may also offset inflammation resulting from intensive training sessions and reduce the delayed onset muscle soreness (DOMS) that is felt after particularly grueling bouts with the iron. Nitric oxide has also been shown to enhance recovery from chronic stress by decreasing tissue breakdown as shown by lower urinary hydroxyproline (UH) levels. The harder we train the more we are at risk of succumbing to performance inhibiting muscular fatigue. By training in the 8-12 rep range (a standard hypertrophy training protocol) with maximally heavy weights we accumulate an abundance of lactic acid, a by-product of anaerobic metabolism and a major limiting factor when it comes to pushing out those extra reps and recovering between sets. By releasing more oxygen into our muscle tissues during training we may negate lactic acid build-up. By relaxing our largest blood vessels and allowing more blood to flow into our muscles, more oxygen is delivered and waste products (including lactic acid) are more efficiently removed. Say Yes to NO As we have discussed, increased blood levels of NO have been shown to improve human performance and in many ways may benefit the muscle hungry among us (although, as is the case for most things, more is not better – always follow dosing instructions as provided on packaging and labels). In addition, the primary precursor of NO, Arginine (and of course Citrulline Malate), has a number of additional benefits of its own, including improved healing times and natural increases in growth hormone (GH). So the next time you go looking for a pre-workout, remember the tenets of Blood Volume Training (BVT) and make sure you are appropriately dosing yourself with efficacious levels of NO enhancing ingredients. Sources Elayan, H, H., Kennedy, B, P., Ziegler, M, G. Selective peripheral regulation of noradrenaline and adrenaline release by nitric oxide. Clin Exp Pharmacol Physiol. 2002 Jul;29(7):589-94. Elam RP, Hardin DH, Sutton RA, Hagen L. Effects of arginine and ornithine on strength, lean body mass and urinary hydroxyproline in adult males. J Sports Med Phys Fitness. 1989 Mar; 29(1): 52-56. International Society of Sports Nutrition Conference Proceedings. Sports Nutrition Review Journal, 1: S1-11, 2004. Kantor, M. Nitric Oxide: the New Hero of human Biology. Psychology Today. [Online] http://www.psychologytoday. com/articles/200502/nitric-oxide-the-new-hero-human-biology Retrieved on 3.2.14 Moretto, M., López, F,J., Negro-Vilar, A. Nitric oxide regulates luteinizing hormone-releasing hormone secretion. Endocrinology. 1993 Nov;133(5):2399-402. Manninen, H. Supplement Performance: Nitric Oxide Boosters. Cutting-Edge Data from ISSN Conference Proceeding. 2005. NobelPrize.org.The Nobel Prize in Physiology or Medicine 1998 [Online] http://www.nobelprize.org/nobel_prizes/ medicine/laureates/1998/illpres/ Retrieved on 3.2.14 Nagaya N, Uematsu M, Oya H, Sato N, et al. Short-term oral administration of L-arginine improves hemodynamics and exercise capacity in patients with precapillary pulmonary hypertension. Am J Respir Crit Care Med. 2001; 163: 887-891. Rettori, V., Belova, N., Dees, W., L., Nyberg, C., L., Gimeno, N., & McCann, S., M Role of nitric oxide in the control of luteinizing hormone-releasing hormone release in vivo and in vitro. Role of nitric oxide in the control of luteinizing hormone-releasing hormone release in vivo and in vitro Proc NatlAcad Sci U S A. 1993 November 1; 90(21): 10130– 10134. Role of nitric oxide in the control of luteinizing hormone-releasing hormone release in vivo and in vitro Suminski RR, Robertson RJ, Goss FL, Arslanian S, Kang J, DaSilva S, Utter AC, Metz KF. Acute effect of amino acid ingestion and resistance exercise on plasma growth hormone concentration in young men. Int J Sport Nutr. 1997 Mar; 7(1): 48-60. David Robson David Robson www.NaturalMuscle.net 33