Lab Matters Summer 2019 - Page 85

the potential to identify any pathogen within a sample, providing a more comprehensive assessment of the infectious agents and any commensal organisms. MRIGlobal has developed PanGIA (Pan-Genomics for Infectious Agents), a sample to sequence workflow capable of detecting pathogens from clinical samples. This fully integrated workflow includes all steps required for sample through library preparation and next-generation sequencing on the Illumina MiSeq and followed by bioinformatics analysis for unbiased pathogen identification. Here we present the results from our analytical validation of the PanGIA system with nasopharyngeal swabs, demonstrating the limit of detection and repeatability of the system with a wide range of pathogen types. We observed an approximate LOD of 10,000-1,000,000 PFU/ CFU per mL for our pathogen surrogates V. cholerae (Gram negative bacteria), S. aureus (Gram positive bacteria), Modified Vaccinia Ankara virus (DNA virus) and Venezuelan Equine Encephalitis virus (RNA virus). The results presented here demonstrate the ability to use the PanGIA pipeline for the detection of clinically relevant pathogens from a clinically relevant matrix. Presenter: Hillary Wood, MRIGlobal, Kansas City, MO, hwood@mriglobal.org Statewide Sample Tracking: Learning from Newborn Screening and Sexual Assault Kit Deployments H. MacIntosh, STACS DNA States have legislation or regulations mandating sample tracking for public health and safety. How can a state program keep track of thousands of samples coming from hundreds of submitters across a large region to ensure timeliness and transparency? Track-Kit sample tracking is being used by thousands of users to track newborn screening specimens and sexual assault kits. Track- Kit is a cloud-based system that is in use or is being deployed in seven states/provinces in North America. More than simply telling you where your samples are, Track-Kit can also: • Alert hospital or lab staff of delays so that they can take action. This not only expedites processing samples, it can save money by avoiding recollection and associated costs (kit, collection, courier and processing). • Give all users the information they need around the clock, including giving the state a global view of the status and location of all kits. • Track inventory to ensure hospitals never run out of kits, which can save time and money. • Increase quality by alerting the collector if a kit has expired. Avoiding using expired kits means the lab gets a quality sample the first time, and avoids requesting a recollection and additional costs. • Meets hospital accreditation requirements as it documents delivery of kits to the lab. This poster shows graphically two use case deployments and how states are benefitting: Newborn screening (NBS): Track-Kit is being used in Ontario by 200 hospitals to track 150,000 specimens a year. The state of Utah will be deploying Track-Kit to 42 hospitals to track 115,000 specimens PublicHealthLabs @APHL APHL.org by the end of 2019. These newborn screening programs know the status of every specimen to ensure timely screening. Specimens must be tested within 48 hours of collection to identify babies who are at risk of serious disease. Before tracking, specimens occasionally went astray, getting lost in the hospital, or getting lost by the courier; the lab may not have known that a baby was missed until days later. Delays in diagnosis and treatment can result in delay in a baby’s development, lifelong serious disease or premature death. Track-Kit is helping Ontario and Utah to avoid delays and meet their timeliness goals. Sexual assault kits (SAK): Many states now have laws specifying that rape kits must be sent to the lab and processed within a certain timeframe and that survivors are informed as to the status of their kits. Track-Kit is being used in Washington State, Arizona and Michigan and is being deployed in Texas and Nevada to track SAKs from collection at the hospital, in law enforcement custody, and delivery to the DNA lab. Using a confidential survivor portal, survivors can access the location and status of their SAK as well as access local, regional and national resources and contact information for their medical facility and law enforcement agency. States are able to maintain a global view of all SAKs to meet their legislative and reporting requirements. Presenter: Heather MacIntosh, STACS DNA, Ottawa, ON, Canada, heather.macintosh@stacsdna.com Workforce Development A New Public Health Laboratory Workforce: The Rise of Millennial Laboratorians A. Wright, Association of Public Health Laboratories After analyzing multiple APHL administered workforce surveys, it is evident that more millennials are joining the Public Health Laboratory workforce but choosing not to remain in the workforce for more than 5 years. This presentation will discuss the important findings from a focus group with PHL millennials on why they choose to work in a PHL, why they choose to remain in a PHL, and trends in their training needs and desires. It will also cover different PHLs models of recruitment and retention practices based on feedback from millennials and lab directors. Presenter: Andrea Wright, Association of Public Health Laboratories, Silver Spring, andrea.wright@aphl.org Early Findings from a National Assessment to Help Shape and Support Public Health and Clinical Laboratory Workforce Development R. Ned-Sykes, S. Strider, Q. Zheng, P. Thomas and Y. Wilkins, Centers for Disease Control and Prevention Challenges in public health laboratory (PHL) and clinical laboratory (CL) workforce development are numerous; but, not all are well documented from a national perspective. The CDC recently began a 3-year initiative — the Workforce Assessment of Laboratory Communities (WALC) — to better enable the development of collaborative strategies to address challenges and needs in laboratory workforce development. As a first step, a literature review was conducted to document the extent and scope of data Summer 2019 LAB MATTERS 83