Lab Matters Summer 2019 - Page 49

APHL 2019 POSTER ABSTRACTS Both targeted and random samples were collected by the four states and shipped to the laboratories in Utah and New Mexico for analyses. Targeted samples were collected from participants who are involved with spraying insecticides to control mosquito populations. Results: The laboratories have successfully validated the biomonitoring method for three metabolites of pyrethroids in urine and have analyzed approximately 250 samples. Results will show how the Utah and New Mexico laboratories worked together and helped each other to assure analytical needs of the consortium are met. Geometric mean and selected percentiles of the pyrethroid metabolites concentrations in urine will be presented. Results of the pyrethroid metabolites from participants in the mosquito abatement districts will be compared with those collected randomly from participants all over Utah. Presenter: Jackie Patel, Utah Public Health Laboratory, Taylorsville, UT, jspatel@utah.gov A Biomonitoring Study to Examine Baseline Exposures to Toxic Metals and Perchlorate in Virginia in a Geographically Representative Population D. Dannouf, S. Wyatt and C. Retarides, Virginia Division of Consolidated Laboratory Services The Commonwealth of Virginia is a geographically diverse state spanning from the Appalachian Mountains to the Atlantic Ocean. Within Virginia are small farming and mining communities, large cities and urban areas, military installations and shoreline fishing and recreation areas; each with its own unique environment. Virginia is rich in natural resources including coal and many minerals including large deposits of uranium in the southern and western regions. Studies have shown drinking water sources may be contaminated with uranium from trace levels to more than twice the EPA allowable maximum contamination limit (MCL) of 30 μg/L.1 Chronic exposure to uranium and other metals can lead to health issues including renal damage. Perchlorate is a highly water soluble chemical and accumulates in leafy green vegetables. It is present in fertilizers, fireworks, munitions and high energy propellants and is ubiquitous in the environment. Chronic exposure to perchlorate can affect thyroid health, resulting in hypothyroidism. Exposure to uranium, barium, cadmium, thallium, lead and perchlorate is being assessed through analysis of urine samples self-collected by volunteers recruited at community colleges throughout Virginia in order to provide a geographically representative population from which to compile data. The Virginia Community College System consists of twenty-three community colleges serving distinct regions of the state. Students, faculty and staff live and work within these regions; therefore, environmental exposures to chemicals are expected to be representative of the area population. Point-in-time urine metal and perchlorate concentrations from convenience samples were evaluated to determine if correlations could be established between several factors. This included geographic location, gender, drinking water source, tobacco use and dietary habits. Presenter: Shane Wyatt, Virginia Division of Consolidated Laboratory Services, Richmond, VA, shane.wyatt@dgs.virginia.gov PublicHealthLabs @APHL APHL.org Wastewater-based Epidemiology to Combat the Opioid Crisis K. Foppe, N. Endo and M. Matus, Biobot Analytics In October 2018, the US Department of Health and Human services (HHS) renewed the declaration that the opioid crisis is a national public health emergency. Since the initial declaration in 2017, there has been an unprecedented growth in awareness of the opioid crisis, but a lack of data to effectively inform public health officials’ decision-making. “Better data” are called for to better understand and suppress the opioid crisis as written in the five-point strategy announced by the HHS. Conventional metrics to assess the scope of the crisis have been limited to drug seizure data, hospital reports, and overdose records. However, these reporting methods are prone to underestimation of the true problem. Better data are inclusive, fast-arriving, and scientifically-sound evidence to allow public health officials to make fast, informed decisions to end this epidemic. Wastewater-based epidemiology (WWBE) is a prominent method of monitoring the consumption of opioid in communities, providing more accurate data near-real time. In the last two decades, WWBE has been successfully used to measure the concentration of opioids, as well as other illicit drugs from raw wastewater influent. WWBE is a semi-real time approach with a turnaround time of days compared to the months and years required for reporting data to be collected. Advancing WWBE, Biobot Analytics measures the concentration of opioid metabolites within a city. Using GIS analysis of the city’s sewer systems, Biobot Analytics selects a representative set of manholes throughout the city to deploy its sampling devices. While the application of WWBE to the upstream sewer network is challenging, it bears significant potential in understanding the true scope of the opioid crisis with finer spatial resolution. Over a 24- hour period, each device collects raw sewage samples which are filtered and loaded through Oasis HLB solid-phase extraction (SPE) cartridges. These cartridges are extracted in the lab and analyzed via HPLC-MS/MS. The method is capable of measuring the concentration of opioids as well as many other illicit and prescription drugs present in wastewater. These data are transformed into consumption data (mg/day/1000 people) as well as milligram morphine equivalent (MME) for opioid compounds. In liaising with cities, these data are used to understand the high- and hidden-risk areas of a city, track consumption trends, and implement efficient interventions in a timely manner. Presenter: Katelyn Foppe, Biobot Analytics, Somerville, MA, katelyn@biobot.io Tips and Tricks for Extracting Perfluorinated Compounds from Drinking Water and Wastewater Using Solid Phase Extraction M. Ebitson and E. Walters, Biotage Perfluorinated compounds (PFCs) are a group of compounds that have been used in a wide array of industrial and household applications, including fabric protectors, non-stick coatings for cookware, coatings for food packaging and in some fire-fighting foams. Due to the strength of the carbon-fluorine bonds within the molecule, PFCs are highly resistant to degradation even when metabolized or exposed to harsh environmental conditions. Their persistence in the environment allows them to accumulate in water sources, particularly those used for consumption. Summer 2019 LAB MATTERS 47