Lab Matters Summer 2019 - Page 47

APHL 2019 POSTER ABSTRACTS Health (NJDOH), partnered with Environmental and Occupational Health and Sciences Institute (EOHSI) from Rutgers University and NJ Department of Environmental Protection (NJDEP), initiated an exposure study to monitor the change of PFNA in serum of local residents after interventions. This study, using a convenience sampling approach, aims to measure PFNA and additional 11 PFAS (listed in CDC Method) in serum for three consecutive years, once per year, for a target population size of 100-120 volunteers. The selection criteria for subjects are residents, between the ages of 20-74, who have lived in the affected communities for at least two years prior to interventions. Drinking water and house dust were also measured for 14 PFAS (listed in EPA Method 537) in first year to confirm the water PFNA met the criteria and the house dust was not contaminated. Questionnaires were administered to collect information on demographics and potential PFNA sources. The first-year study was completed with recruiting 120 subjects and 105 home visits were made. Among the collected samples, 120 sera, 105 tap waters, and 74 house dust samples were analyzed at PHEL-NJDOH. The average PFNA serum level from the recruited volunteers (n=120) was ~4 times higher than the national level (CDC NHANES in 2013-2014). All PFNA water levels were lower than NJDEP PFNA drinking water standard (<13 ng/L) and PFNA in house dust for all homes was not significantly higher than the levels found in control homes (n=20). The second-year study is ongoing; ~86 serum samples were collected to date, and 45 sera were analyzed. Results showed on average 13% reduction of PFNA was observed from Year 1 to Year 2 from the same 45 subjects. The second-year serum collection will be completed by March 2019. The half-life of PFNA will be estimated after completion of the third collection. In addition, estimated PFNA half-life will be obtained from individualized Physiologically-Based Pharmacokinetic Modelling (PBPK) that incorporates exposure estimates based on reported water consumption for each subject while they resided in the affected area. This study will provide useful scientific background to help design future biomonitoring studies to determine cross- sectional PFAS levels and track the trends of PFAS body burdens. Presenter: Chang Ho Yu, New Jersey Department of Health, Ewing, NJ, Legionella spp. Monitoring in New York City’s Water Distribution System E. Omoregie 1 , A. Szczerba 2 , J. Novak 1 , S. Hughes 1 , A. Capetanakis 2 , S. Feud 2 , J. Rakeman 1 ; 1 New York City Public Health Laboratory, 2 New York City Department of Environmental Protection Legionella, primarily Legionella pneumophila serogroup 1, is the causative organism for legionellosis and has received increased attention as a result of several highly publicized national outbreaks of Legionnaires disease. Legionella spp. are ubiquitous in aquatic systems and have been shown to be present in source and distribution waters. The presence of Legionella spp. in NYC’s drinking water distribution system has not been systematically investigated. Therefore, the NYC Department of Environmental Protection (DEP) and NYC Department of Health and Mental Hygiene (DOHMH) conducted a joint one-year longitudinal study to determine the prevalence and distribution of Legionella spp. in NYC source water and the distribution system. Twelve monthly sampling events took place between November 2017 and October 2018. A total of 255 water samples were PublicHealthLabs @APHL collected from 19 locations each month, representing water from NYC’s watersheds. Samples from different stages of water treatment were tested, including untreated source water, prefinished water and finished waters from various distribution sites including those with historically low residual chlorine levels and longest residence times. Physicochemical and microbial characteristics were measured for all samples and Legionella detection was conducted using PCR and culture methods. All water samples met applicable (EPA and NYS) water quality guidelines for physiochemical and microbiological parameters, and. Legionella spp. were infrequently detected by culture. Legionella spp. DNA was detected by PCR in all (23/23) untreated source water samples, but Legionella DNA detection in prefinished and finished water samples was variable. In total, 88% (202/231) of the water samples had detectable Legionella spp. DNA, which is unsurprising given Legionella spp. are ubiquitous in aquatic environments. Despite frequent detection of Legionella DNA throughout the distribution system, only 2.5% (6/231) of samples were culture positive. L. bozemanii (50 CFU/100 mL) was recovered in the same source water site on two occasions and L. pneumophila (1 - 20 CFU/100 mL) was recovered from the same distribution site on four occasions. Results did not indicate a correlation between L. pneumophila recovery and physicochemical changes within the distribution system. However, the positive distribution site was in the midst of an ongoing new main replacement and street construction project. Although Legionella DNA was detected throughout the distribution system, the lack of recoverable Legionella in samples collected in this study suggest these Legionella were non-viable and that the current disinfection practices are effective in protecting against the presence of Legionella spp. in New York City’s water supply. Presenter: Enoma Omoregie, New York City Public Health Laboratory, New York, NY, Iowa Private Well Survey: Partnerships for Public Health and Emerging Contaminants Research M. Schueller, M. Pentella and A. Mattson, State Hygienic Laboratory at the University of Iowa The quality of water sources from which people receive drinking water is of utmost importance to the health of the served population. Private well water consumers in the United States are an under served and under-protected population relative to public health. It is estimated that more than 13 million households and one out of nine Americans rely on private wells for drinking water. Private wells are generally not regulated for routine testing and monitoring them for water quality assurances is at the discretion of the private well owners. Inconsistent testing requirements and lack of regulation for private wells presents challenges for public health in rural communities. The State of Iowa has approximately 110,000 private wells that provide drinking water to nearly 300,000 people living primarily in rural settings. Private wells in Iowa are not regulated for minimum quality requirements for any chemical, biological or microbiological compounds. The State Hygienic Laboratory at the University of Iowa (SHL), in strong partnership with county public health departments, initiated the Iowa Well Survey (IWS) in the fall of 2017. The IWS is a sustained, statewide effort to reach more private well owners and present them with an opportunity to have their well(s) tested, free of charge. SHL has Summer 2019 LAB MATTERS 45