Lab Matters Summer 2018 - Page 78

APHL 2018 Annual Meeting Poster Abstracts Presenter: Erik Reisdorf, MPH, Wisconsin State Laboratory of Hygiene, Madison, WI, Phone: 608.224.4261, Email: erik.reisdorf@slh.wisc.edu Reduced Viability of Candida auris and Other Candida Species from MALDI-TOF Extractions A. Sterkel, A. Bateman, A. Valley, D. Warshauer, Wisconsin State Laboratory of Hygiene, Madison, WI Candida auris is emerging as a health care associated and often multidrug-resistant, global public health threat. The ability of this yeast to persist in the environmental and colonize skin has made it challenging to eradicate and complicated efforts to prevent transmission in health care settings. C. auris is capable of causing invasive infections and is associated with increased mortality. There continue to be reports of laboratory misidentifications of this previously rare organism. Matrix-assisted, laser desorption/ ionization time-of-flight (MALDI-TOF) mass spectrometry is an accurate and relatively cost effective method for identifying this emerging species of Candida. However, there is a lack of data about C. auris viability after the various preparation protocols for MALDI-TOF testing. In an effort to evaluate and improve biosafety for laboratorians we investigated the viability of Candida after different MALDI-TOF extraction methods. We tested 10 isolates of C. auris and 15 isolates of other Candida species with three different MALDI-TOF extraction methods: on-plate extraction with formic acid; 50% ethanol in a tube; and tube extraction with formic acid and acetonitrile. After spotting the Bruker MALDI-TOF target with each preparation, overlaying with matrix and allowing to dry, a flocked swab dampened with RPMI broth was used to remove the dried preparation. Swabs were inoculated into RPMI broth and onto Sabouraud dextrose agar and observed for growth after 24 and 48 hours of incubation at 37 C. No growth was observed with any species after treatment with 50% ethanol or after any step in the tube extraction with formic acid and acetonitrile. On-plate extraction with formic acid greatly decreased the viability (average: 99% decrease in colony counts). And, colonies were only observed with C. albicans. These data indicate that the ethanol treatment and tube extraction methods are sufficient to kill C. auris and other Candida species prior to MALDI-TOF analysis. Presenter: Alana Sterkel, PhD, SM(ASCP), Wisconsin State Laboratory of Hygiene, Madison, WI, Phone: 608.224.4253, Email: alana.sterkel@slh.wisc.edu Informatics Electronic Test Order and Result K. Higginbotham 1 , M. Meigs 2 , D. Shirazi 2 , S. Crowe 3 , G. Click 4 ; 1 Alabama Department of Public Health, Montgomery, AL, 2 Association of Public Health Laboratories, Silver Spring, MD, 3 Florida Department Of Public Health, Tallahassee, FL, 4 J Michael Consulting, Atlanta, GA With a surge in Zika testing in 2016 and typical laboratory workflows resulting in over 60 steps to test a single specimen, the need became clear for a new approach. APHL has been funded by ONC to enhance existing Electronic Test Order and Result capabilities and functionalities across laboratories with the advent of a Lab 76 LAB MATTERS Summer 2018 Web Portal. Designed to streamline testing and accommodate high-volume requests in testing with emerging infectious disease threats, the portal allows epidemiologists to prioritize testing within laboratories, grant approval and communicate with the submitter as needed. This poster will feature some of the unique capabilities of the Lab Web Portal and will demonstrate how its use can improve laboratory testing processes. Presenter: Ke Fvv&F&FW'FVBbV&Ɩ0VF'W&VRbf&FFV6wFvW'S33B#bsCVâVF憖vv&FG7FFRW0WfWFb( 2f&F72FV66767F6R&w&ХbW6&F"֖FB6FW'6B"W'&6766FbV&Ɩ2VF&&F&W26fW"7&r@7&V6rV&W"bV&Ɩ2VFvV6W2B&v旦F0&RGFVFrF'VBWGv&2F6&RFFFB&RW'FV@FV&Ɩ2VFFV66r7&72fVFW&B7FFR77FV0B&WGvVVV&Ɩ2B&fFR'FW'2vWfW"FR6VvW0bVVFr7V6FFW6vRFW&2br&6&V6r66V7W2r7FVFW'26&FFp7FfFW2G&6rB&W6fr77VW2Bv&rv7@w&VVBW&V7B66VGVRgFV憖&BFR7V66W76gVVVFFbFFW6vR&V7G22ffW&V@f&F72f'GVBW'6FV66767F6Rf"FR7@FVV'2FVV&Ɩ2VFVWBFW6RVVG2F27FW"vW֖RFRWfWFbFV66767F6RfW"FRV'2@r2VVBV&Ɩ2VF&&F&W2BvV6W0VFW'7FBfvFRBVVBVV7G&2FFW6vRF@W6W26RVffV7FfR7FF&G2&6VBWFG2&W6VFW#fW76W766FbV&Ɩ2VF&&F&W26fW"7&rBS#CCR#sSRVàfW76W&pW6rF2Ff&f&F727G&FVwbW"6WW&BW&&vR"7BFƦWb"766FbV&Ɩ2VF&&F&W26fW"7&rB"֖6V67VFrFFt2V&Ɩ2VF&&F&W2&R&WV&VBFVWB7&V6pf&F72&WV&VVG2FWf6R7'V6FV662&WBFV fW&f&F727G&FVwFV662&Vv&FrĔ2Ww&FW0"&W6VVG277FVFW&W&&ƗGBFVfrgV7F&WV&VVG2&RgFVFW2Fff7VBB&WV&R&&F&W2FfR6&VV6fRVFW'7FFrB76W76VBbFV 7W'&VBf&F726&ƗFW2FVFW6RVff'G2FPf&F726Vb76W76VBFv2FW6vVBFV&&F&W076W727G&VwF2Bv2FV"f&F7266G'FG&W76r7&F6"W&F&V2&&F'66V7W&RFR&ƗGbG2&&F'f&FvVV@77FVĔ2"FW"6gGv&RFVWB&62&WV&VVG2v7@( 2( &WV&VVG2f"V&Ɩ2VF&&F'f&FvVVB77FV( F27FW"vW֖RFR&7F6f&F72F2ffW'2FG2V&W'2FVFVPf&VBFV662FV"f&F727FfFW2B&V7G2&W6VFW#fW76W766FbV&Ɩ2VF&&F&W26fW"7&rBS#CCR#sSRVàfW76W&pV&Ɩ4VF'0&p