Lab Matters Summer 2018 - Page 67

APHL 2018 Annual Meeting Poster Abstracts among all three beaches. Bacterial load of 0.5% at Bradford, 0.8% at McKinley and 0.04% at South shore beach was observed. However, top five abundant microbial strains (Hot Spots) were found in South shore beach, followed by McKinley and Bradford beach. Heatmaps were generated for bacteria, fungi and protist using relative abundance of organisms. Principal Component Analysis based on covariance between bacterial community among each pair of samples demonstrated close clustering between Bradford and McKinley samples indicating greater microbiome similarly between these two areas whereas South Shore samples were clustered away from those. Community resistome profiles demonstrate presence of genes associated with Aminoglycoside resistance in all samples, indicating possible environmental reservoir of Aminoglycoside resistance developed by Gram-negative bacteria. Conclusion: Metagenomic sequencing approach to explore microbial communities offered critical inside, both on ecological and public health perspectives and underscore the crucial need for more frequent microbiome analysis of waters people encounter on the shoreline. Presenter: Sanjib Bhattacharyya, PhD, City of Milwaukee Health Department Laboratory, Milwaukee, WI, Phone: 414.286.5702, Email: L. Sanders, M. Rickard, K. Riddle, C.L. Truong and M. Hung-Fan, Contra Costa Public Health Laboratory, Martinez, CA Syphilis infection rates are rising in California, especially in high risk populations. Management of patient outcome and infection control requires prompt and accurate identification of early and active syphilis infections. Traditionally, laboratories screen for antibodies common to infection, but not specific to Treponema pallidum; reactive specimens are tested for T. pallidum-specific antibodies. Seeking increased automation and specificity, new technologies make a case for algorithm reversal: screen for treponemal antibodies and confirm with non-treponemal antibodies. We evaluated traditional and reverse testing algorithms that incorporate the Bio-Rad Laboratories BioPlex 2200 Syphilis Total and Rapid Plasma Reagin (RPR) assay, which detects treponemal and non- treponemal antibodies. In our current traditional algorithm, we screen with RPR; reactive specimens reflex to quantitative Venereal Disease Research Laboratory test (VDRL) and Treponema Pallidum Particle Agglutination assay (TPPA). We evaluated 359 samples by manual RPR (Arlington Scientific, Inc.) and BioPlex 2200 Syphilis Total &RPR. Samples reactive by manual RPR reflexed to VDRL (Becton Dickinson) and TPPA (FujirebioSERODIA). We initially tested 203 samples with each treponemal and non-treponemal assay (excludingVDRL). We tested 156 samples with each non-treponemal test (excluding VDRL) and the BioPlex SyphilisTotal; but not TPPA. For the 65 samples reactive with manual RPR, BioPlex RPR, or both, we determined titers with non-treponemal assays (VDRL, manual RPR and BioPlex RPR). BioPlex RPR data have positive and negative agreement of 92% and 95%, respectively, with our manual RPR data. BioPlex Syphilis Total data have positive and negative agreement of 99% and 100%, respectively, with our manual TPPA data. Compared to our current algorithm, the algorithm that screens with the BioPlex RPR has a sensitivity of 92% and specificity of PublicHealthLabs @APHL Presenter: Kelly Riddle, Contra Costa Public Health Laboratory, Martinez, CA, Phone: 925.370.5775, Email: Pseudomonas aeruginosa Alters Staphylococcus aureus Sensitivity to Antibiotics G. Orazi, K. Ruoff and G. O’Toole, Geisel School of Medicine, Dartmouth College, Hanover, New Hampshire Cystic fibrosis (CF) patients have impaired mucociliary clearance and thick mucus, which promote respiratory infections. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent respiratory pathogens in CF infections. Both organisms are associated with poor lung function and patient outcomes. In addition, P. aeruginosa and S. aureus form biofilms in the airway, which cause chronic infections that are recalcitrant to antibiotic treatment. 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