APHL 2018 Annual Meeting Poster Abstracts independent testing (CIDT) but no E. coli O157:H7 was recovered via traditional culturing techniques. This subset of samples includes the reference (index) patient and an environmental sample of horse manure. Presenter: Kelly Oakeson, PhD, Utah Public Health Laboratory, Taylorsville, UT, Phone: 801.965.2400, Email: firstname.lastname@example.org Comparison of Sequence-based Salmonella Serotyping Methods for Use in a State Public Health Laboratory N. Florek, S. Wagner, K. Gundlach, A. Jaedike, A. Bateman and D. Warshauer, Wisconsin State Laboratory of Hygiene, Madison, WI Background: Public health surveillance and outbreak investigations of Salmonella are historically monitored through Salmonella serotyping. With the introduction of whole genome sequencing (WGS), public health laboratories are looking to sequencing to replace conventional testing by producing a highly accurate analysis of the genotype. However, in order to use WGS in a public health laboratory the approach must undergo a rigorous validation to meet Clinical & Laboratory Standards Institute (CLSI) standards. Results: Agreement was highest between conventional serotyping and SISTR at 93%, which improved to 95% after repeating conventional serotyping on five discordant isolates. Agreement between SISTR and SeqSero was lower at 88%. Agreement was lowest between conventional serotyping and SeqSero at 85%, but improved to 87% after repeating conventional serotyping on 5 isolates as above. Between all 3 methods there was an overall agreement of 85%. Conclusion: Both sequence-based Salmonella serotyping methods provided results at a higher accuracy than conventional antisera. Agreement between the sequence methods led to the repeated analyses on 5 samples that had been incorrectly serotyped. SeqSero’s difficulty in distinguishing closely related serotypes or subspecies accounted for most of the disagreements between the sequence-based methods. SISTR’s approach of using both O, H1 and H2 antigen gene detection alongside a core genome MLST analysis appears to allow the most accurate serotype determination. Presenter: Nicholas Florek, PhD, MPH, Wisconsin State Laboratory of Hygiene, Madison, WI, Phone: 608.224.4331, Email: email@example.com Global Health A Quality-Integrated and Leadership-Focused Approach to Biorisk Management Training In West Africa J. Alam, C. Asbun, B. Karlstrand, M. Mar, D. Roberts, S. Shearrer, L. Presser and S. Altman, MRIGlobal, Gaithersburg, MD The high rate of Ebola virus infection among West African laboratory workers during the 2014–2016 epidemic demonstrated a need to PublicHealthLabs @APHL APHL.org Presenter: Jane Alam, MRIGlobal, Gaithersburg, MD, Phone: 240.361.4043, Email: firstname.lastname@example.org An International Collaboration to Develop Laboratory Leadership Competencies to Strengthen Laboratory Workforce Development in Support of Global Health Security J. Isadore, S. Dittrich 2 ; 1 Public Health Works, LLC, Holliston, MA, 2 Association of Public Health Laboratories, Silver Spring, MD In 2014 the Global Health Security Agenda (GHSA) was launched to accelerate progress towards global health preparedness and to support capacity-building efforts. The GHSA Workforce Development Action Package emphasizes the need for rigorous and sustainable training programs including practical hands-on experience for public health professionals. Currently, laboratory science training programs vary widely across the globe; however, a component consistently absent in these curricula is leadership training. In low- and middle- income countries there are limited stand-alone opportunities for laboratory-specific leadership training. Thus a majority of laboratory managers learn on-the-job through experience and if available, mentorship rather than formal training. Core competencies for laboratory leadership are critically important to improve training opportunities by informing curriculum development and guiding professional development. The need for a comprehensive global framework including competency-based and laboratory-specific leadership training and mentorship will allow countries to move forward towards meeting the GHSA workforce development goals. Global consensus and publication of laboratory leadership competencies will encourage standardization of laboratory leadership learning approaches and encourage their use by national and international implementing agencies. To this end the Association of Public Health Laboratories (APHL), the US Centers for Disease Control and Prevention (CDC), the European Centre for Disease Prevention and Control (ECDC), the Food and Agriculture Organization of the United Nations (FAO), the World Organization for Animal Health (OIE) and the World Health Organization (WHO) have partnered to develop a Laboratory Leadership Competency Framework and training package for a Global Laboratory Leadership Summer 2018 LAB MATTERS 59 Methods: As part of CLSI validation, we evaluated two sequence based Salmonella serotyping tools, the Salmonella in silico Typing Resource (SISTR) and SeqSero for use at the Wisconsin State Laboratory of Hygiene. We compared serotype identification of 255 Salmonella enterica isolates using conventional antisera, SISTR and SeqSero. strengthen biorisk management (BRM) in local, national-staffed laboratories. A systems approach for integrating biosafety and biosecurity within an institute or facility, BRM is typically taught as a set of policies and procedures distinct from other clinical laboratory training. A common underlying assumption is that, once trained, participants will develop BRM programs for their institutions with minimal guidance and harmonize them with their existing laboratory quality management systems (LQMS). This assumption is problematic in West African laboratories. Many labs currently lack fully developed LQMS. The labs also typically lack st rong leadership and support required for successful implementation and sustainment. With these deficiencies in mind, a unique curriculum was created integrating LQMS training into a standard BRM training curriculum and specifically targeting institute directors, deputy directors, stakeholder ministry representatives and laboratory management personnel. The training curriculum was implemented in three countries; Liberia, Sierra Leone and Guinea. Case studies for each are presented along with their individual challenges and strengths. It is important to remember that while our models are state and local health departments in the USA, the structures that allow for efficient operations at these facilities are not always available, or differ in West African countries.