Lab Matters Summer 2018 - Page 60

APHL 2018 Annual Meeting Poster Abstracts recovery of YE from stools positive by the GI panel and subsequently to evaluate these isolates further using laboratory epidemiological methods for outbreak investigating. Methods: Stools preserved in maintenance media that had YE detected by the GI panel were either directly cultured to CIN agar plates incubated at room temperature (RT) for up to 5 days or 500 Β΅l placed into sterile PBS (10 ml) and incubated at 4Β°C (cold enrichment) for up to 2 weeks with incremental subcultures to CIN agar. Isolates detected from these stools were subsequently tested by pulsed-field gel electrophoresis (PFGE) using XbaI restriction enzyme and whole genome sequencing (WGS) (Illumina MiSeq). Cluster analysis of PFGE profiles and WGS single nucleotide polymorphism (SNP) analysis were performed using BioNumerics 7.6. Results: Twenty-six stools positive by the GI panel were cultured for YE. YE was recovered from 14 stools (53.8%) following RT incubation and a total of 21 stools (80.1%) following cold enrichment. Eighteen of these isolates were further evaluated using PFGE and WGS. 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