Lab Matters Summer 2018 - Page 54

APHL 2018 Annual Meeting Poster Abstracts Method Evaluation and Qualification for an ABSciex 5500 Triple Quadrupole Mass Spectrometer J. LaPalme, Y. Wang, O. Adair and R. Dixon, South Carolina Public Health Laboratory, Columbia, SC The timely and accurate testing for exposure to toxic substances is a critical component of the Public Health Laboratory’s mission. The Centers for Disease Control and Prevention Laboratory Response Network for Chemical Threats (LRN-C) leads the effort in ensuring emergency response capabilities. In this poster we will highlight the method performance of an organophosphate nerve agent metabolites in urine assay by ultra-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Due to South Carolina DHEC Public Health Laboratory’s chemistry division instrumentation reaching the end of its life cycle and vendor support, the previous ABSciex 4000 triple quadrupole mass spectrometers were updated to the SCIEX 5500 model with support from the LRN-C program. In order to maintain testing capabilities, the analytical methods were transferred and rigorously evaluated with a thorough CLIA validation protocol.The LC-MS/MS assay for organophosphate nerve agent metabolites in urine was one of the first methods to be transferred from the ABSciex 4000 MS/MS to the SCIEX 5500 MS/MS. The analytical Chemistry Supervisor and the Chemistry Division Director agreed upon the method validation protocol to be implemented. The following method characteristics were examined: accuracy, precision, method comparison, reportable range, analytical sensitivity, analytical specificity, carryover, matrix effects and reference range. Acceptance criteria were established to ensure method robustness and accuracy. The validation study was carried out over a three week period. Six different laboratory chemists participated in the performance evaluation producing 20 calib &F7W'fW2FW&R&R6Ɩ'&FWfV0W2BVƗG6G&WfV26VFrG&&ǗF667W&7v2FWFW&֖VB'6&rFR67VFVBVfVRvFFRG'VRF&vWBfVR&V66v2FWFW&֖VB'67VFrFRW&6VB6Vff6VBbf&FT5bbFRVƗG6G&6RV7W&VVG2BF&VRFffW&VBWfV27&70FRǗF6V7W&VVB&vR&FG&FBFW"F&V66FV7G&FVBW72Fb#RT5bWFB6&6খffVB&WfW6ǒ76VB6W2f"V6ǗFRFRFFv2GFVB'ǗFRf"V6&Vw&W76Ǘ62G&VffV7G0vW&RWfVFVBW6rF&VR66VG&FWfV27&72BVG0b6RG&FR&VfW&V6R&vRv2WfVFVBW6r#vVVR&ƖFVBVW&R6W2F2VFǗFR76fƖFFWBFR66WF6R7&FW&W7F&Ɨ6VBV&ƖrFP&&F'F6FVR&fFr67W&FRFW7FrFFR6VGb6WF6&Ɩ&&F&W2VVBF6FVǒWFFR7627G'VVFFB&6W76W2&FW"F7V66W76gVǒG&6F&WGvVVFV6VW2WFBWfVFw2f"7FfbG&r@W&f&6RfƖFF6ƖBf6FV7W&RvVƗGFW7B&W'Fr&W6VFW#W2R6WF6&ƖV&Ɩ2VF&&F'6V&42S2ビb3cbVà֦FV262v`vV֖26֖&GbVvVWV6FVBg&Х&WFRF&rb7F&V֗6RV&r77FV06WFR'7G&7BfV7FW2F6V6RsRS "EDU%27VW"#FRU( 2vFW"6F֖Bf&FFt4BBF&VRWr6F֖B&fW3W&fV&7FP7VfFRd2W&fV&7F26Bd@VvV&rv&FVW"BR2Vf&VF&FV7FvV7v6wFD0FRWfVBbGW&FVF"VFVFvFW 6F֖F6FVBV6BVffV7FfR&W76R27'V6FƖ֗FrFR7BvFW"77FV2BV&Ɩ2VF&W7FpF6F֖FWfVB&WV&W267W&FRf&FFPGW&RbFR6F֖BBrF&W&ǒG&VBBFRvFW 6F֖Bf&FFt4B2G֖2BfV&P&W6W&6RFW6vVBF&fFRF2f&FF&W7FW'2@FG&W72Wr&62B77VW22FW&6Rt4B26V7W&RƖRFF&6RFB6F2fFf&F7V62ǗF6WFG2G&涖rvFW"Bv7FWvFW"G&VFVB&6W76W0BVF6f&F&&G6F֖G2b66W&f"G&涖rvFW"Bv7FWvFW"77FV227V6F2Ff6ƗFFW2FRF76V֖FbfFf&FFB6&RW6V@FV6VFW2&W&Rf"&W7BFB&V6fW"g&Ц6FVBW&RvR&fFRfW'fWrbFRFvƖvFp6RbG2VVRfVGW&W2BW6W2B&W'B&V6VBWFFW2FFRFF&6Rt4B267FFǒ&VrWFFVBvFf&F&&G6F֖G2FVWFƗFW2BFW"W6W'2&W7@VffV7FfVǒBVff6VFǒFG&涖rvFW"Bv7FWvFW 6F֖F6FVG2FR7B&V6VBWFFW2FFRFfPf7W6VBFFrWr&fW2VW&vr6F֖G2bFPw&VFW7B66W&f"WRW&fV&7FR7VfFRd2BW&fV&7F26BdfRvVBBGFVF&V6VFǒGVRFFV"W'67FVBGW&RFRV&G@Vf&VBBFV"FVFGfW'6RVFVffV7G2VRFRU&VV6VBWrVFGf6'#bvW&rFP&V6VFVBWfV2G&涖rvFW"g&CBf"d@#Bf"d2FsB6&VBdBd2fvpFR&VV6RbF2WFFVBVFGf6't4B&fW2fP&VVFWfVVBf"FW6R6F֖G2Bv&RF67W76V@W&RFFFǒFW"6F֖BFB2v&W&VBB`GFVF&V6VBV'2B2"66W&f"vFW"77FV02VvVWVVvV2&7FW&VFBF&fW0v&vFW"B66W6R6WfW&RWVGRVpfV7FFB2v'FƗG&FRWF3RW7V6ǒখV6&֗6VBVRFVWFƗFW2B&W7FW'0FG&W72F26F֖Bt4B&fRv27&VFVBB26F67W76VB&W6VFW#vV&vRv&FVW"U2Vf&VF&FV7FvV7v6wFD2S#"#s"crVàv&FVW"vV&vTWv`66W"BU2Vf&VF&FV7FvV7f6ƗFW0'W&2BƖV'&U2Vf&VF&FV7FvV7v6wFD0g&FRV&ƖW7BF2bV&Ɩ2VFVf&VF&FV7Fআ2&VVgVFVFF&WfVFrF6V6RBV7W&rVFVFg&6rBFR'&B7G&VWBVF6&FVBG&涖rvFW"W'6W6G&FV7FrFPVf&VB2&VV6&RV&Ɩ2VFgV7FFR6R0G'VRFFvRFRV&Ɩ2VF6VGFRR2vW V&Ɩ4VF'0&p