Lab Matters Summer 2018 - Page 52

APHL 2018 Annual Meeting Poster Abstracts 100–200 volunteers, between the ages of 20 and 74, from the residents who have lived in the affected communities during past three years. Drinking water and house dust are also measured for 14 PFASs listed in EPA water testing method (Method 537) in first year. Questionnaires are administered to collect information on demographics and potential PFNA sources. The subject recruitment was begun in summer of 2017. To date, 85 subjects were recruited and home visits were made, including questionnaire survey, blood draw and water/dust collection. Among the collected samples, 65 sera, 46 tap waters and 36 house dust samples were analyzed at PHEL in NJDOH. The results showed serum PFNA ((GM [95% CIs]; 4.10 [3.09-5.44] ng/mL, n=65) were higher than a national average (0.68 [0.61-0.74], n=2168) from the latest National Health and Nutrition Examination Survey (NHANES). For drinking water, all samples were lower than reporting limits (5 ng/L). Some PFASs were detected (>10 ng/g) in house dust. We will complete the first- year recruitment and sample analyses by the summer of 2018 and repeat serum analyses for the same subjects at year 2 and 3. The obtained data will be used to evaluate whether the intervention is effective and to identify significant sources of PFNA exposure. This study will demonstrate a biomonitoring study is a useful tool to assess effectiveness of intervention on community exposure to PFAS-contaminated drinking water. In addition, this study approach will provide a foundation for future researches in other PFAS- affected communities across the US. Presenter: Zhihua (Tina) Fan, PhD, New Jersey Department of Health/PHEL/ECLS, Ewing, NJ, Phone: 609.530.2803, Email: tina.fan@doh.nj.gov An Assessment of Serum for Six Perfluoroalkyl Substances from a Small Human Population L. Blum, S. Donovan and D. Schiller, NMS Labs Six perfluoroalkyl substances (PFAS) used by the U.S. EPA to assess drinking water under the Third Unregulated Contaminant Monitoring Rule (UCMR3) were tested in 151 randomly selected de-identified serum samples. PFASs are a group of chemicals used in a variety of industries and consumer products. As a result of their use, these persistent chemicals can be found in drinking water. The six PFAS contaminants tested in drinking water by the U.S. EPA under UCMR3 include perfluorobutanesulfonic acid (PFBS), perfluoroheptanoic acid (PFHpA), perfluorohexanesulfonic acid (PFHxS), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA) and perfluorooctancsulfonic acid (PFOS). The serum samples selected for testing had unknown exposures to these six PFASs. Sixty-four (64) of the samples tested were from females and 87 from males. The samples were from individuals ranging in age from 1 to 90 years (average = 40.7 Β± 20.7 years; median = 41.5 years) and were submitted from 24 different U.S. states. The quantitative assay used to test for the six PFASs in these serum specimens consisted of HPLC separation with negative-ion electrospray tandem mass spectrometry (LC-MS/MS). The samples were prepared for analysis by adding analyte specific 13Carbon isotopes as internal standards with subsequent protein precipitation. The analytical measurement ranges (AMR) were 0.05 to 10 ng/mL for PFBS, PFHpA, PFHxS and PFNA; and 0.5 to 100 ng/mL for PFOA and PFOS. This population of specimens (n=151) with unknown exposures to these substances showed the following respective median and 97.5th percentile values as measured for the linear components of PFBS (<0.05, 0.06 ng/mL); PFHpA (<0.05, 0.47 ng/mL); PFHxS (0.99, 5.75 ng/ 50 LAB MATTERS Summer 2018 mL); PFNA (0.42, 1.44 ng/mL); PFOA (1.12, 4.13 ng/mL); and PFOS (1.77, 11.5 ng/mL). The values determined in this investigation were similar to other studies that measured these substances in the general population. This assay can be used in biological monitoring of serum for the six PFASs tested in drinking water under EPA UCMR3. 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