Lab Matters Summer 2018 - Page 51

APHL 2018 Annual Meeting Poster Abstracts exposures in urine and blood from the state population. The two quadrupoles (Q1 and Q2) are separated by a collision/reaction cell, which allows for effective removal of interfering polyatomic ions when operated in tandem MS/MS mode. The instrument may also be operated in traditional or single quadrupole mode, with Q1 acting as an ion guide for enhanced signal-to-noise when measuring analytes that have very low abundances, such as uranium. By coupling a high-performance liquid chromatograph (HPLC) to the ICP-MS, the NH PHL has also developed a method for measuring six arsenic species in urine with limits of quantitation as low as 0.5 ppb, which allows for very low-level background exposures to be determined for surveillance study applications. The analytical flexibility and advanced performance of triple quadrupole ICP-MS methods are suitable for determining a wide array of analytes of interest for biomonitoring public health studies and may be particularly useful for studies that investigate changes in toxic metals exposures as the result of industrial practices or natural disasters, as well as studies that track the effectiveness of remediation techniques on reducing human exposures. Presenter: Kimberly Aviado, PhD, New Hampshire Public Health Laboratories, Concord, NH, Phone: 603.271.9072, Email: kimberly.aviado@dhhs.nh.gov C. Dingman, K. Aviado, J. Schneider and C. Bean, New Hampshire Public Health Laboratories, Concord, NH Perfluoroalkyl and Polyfluoroalkyl Substances (PFASs) are a group of man-made chemicals that are widely used in consumer products. PFAS compounds can remain inside the body for an extended period of time and their health effects are still unknown. The Biomonitoring Program at the New Hampshire Public Health Laboratories (NH PHL) plans to implement a statewide surveillance study to assess exposure of New Hampshire residents to a variety of chemicals that may be present in the environment, including perfluoroalkyl substances. The NH PHL has validated a method for the analysis of 12 PFAS (six perfluorocarboxylates, three perfluorosulfonates and three perfluorosulfonamides) in serum for this study. The method uses high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) and sample preparation is simple, rapid and semi-automated. A 96 well plate utilizing protein precipitation and phospholipid removal is able to quickly prepare large batches of samples for analysis. In addition, an on-line solid phase extraction (SPE) method with reusable SPE cartridges has been developed to supplement sample preparation capacity. The method validation demonstrated accuracy, precision and linearity from 0.1–50 ng/ mL over twenty independent analytical runs. Matrix-matched quality control materials were prepared in-house and characterized during the validation. NIST standard reference materials (SRM 1957 and 1958) were also analyzed throughout the validation to verify method accuracy. The method requires only a small sample volume (50 µL) and is highly sensitive, with limits of detection ranging from 0.03 to 0.09 ng/mL, making it suitable for large-scale biomonitoring studies. An important goal of the statewide surveillance study is to improve the health and wellness of New Hampshire’s citizens with robust PFAS exposure data. Presenter: Carleen Dingman, New Hampshire Public Health Laboratories, Concord, NH, Email: carleen.dingman@dhhs.nh.gov PublicHealthLabs @APHL APHL.org S. Du, N. Patterson and C.D. Riker, New Jersey Department of Health, Ewing, NJ Polychlorinated biphenyls (PCBs) are persistent pollutants and detectable amounts are constantly found in blood of most populations that have been examined. As part of efforts of New Jersey Department of Health State Biomonitoring program, forty ortho-substituted PCBs are measured in 300 serum samples collected from New Jersey residence using high-resolution gas chromatography/isotope-dilution high-resolution mass spectrometry (HRGC/ID-HRMS). The objectives of this study are to characterize the concentration level and pattern of PCBs in New Jersey residence by age, gender and ethnicity and identify major exposure factors. Based on the completed analysis of 150 samples so far, the median concentration of the sum of 40 PCB congeners (SPCBs) was 110 ng/g lipid, with a 90th percentile of 255 ng/g lipid, minimum of 13 ng/g lipid and maximum of 819 ng/g lipid. As the sample analysis continue going, the reported numbers will be subject to change later. With the completion of more samples in the subsequent months, we will compare the SPCB concentrations in New Jersey Residence to the NHANES 2003–2004 results stratified on age and race. In addition, the relative contribution of individual congeners to SPCBs will be examined and their variation among different age and race group will be elucidated. Furthermore, factor analysis modeling will be applied to identify the co-varying congener patterns to reveal the contributing exposure factors for the observed variation of measured PCB congeners in collected samples. Presenter: C. David Riker, New Jersey Department of Health, Ewing, NJ, Phone: 609.530.8728, Email: collin.riker@doh.nj.gov A Community Biomonitoring Study to Assess PFASs Body Burdens from PFNA-Contaminated Drinking Water in New Jersey C.H. Yu 1 , C. Weisel 2 , S. Alimokhtari 2 , S. Minchala 2 , D. Riker 1 , Z. Fan 1 ; New Jersey Public Health and Environmental Laboratory, Ewing, NJ, 2 Rutgers University, New Brunswick,NJ 1 In 2012, elevated perflu ܛۛۘ[XXY JKHX[[XYB[ZX[\Yو\H[KY[ܛ[[X[\ TK\]XY[XX]\\[\[]]H[[][؛ܛ˜[\\ܙ[][]Y\[]\^H KH\\BXH[H[\ۛY[[[H[X[][]Y[B[X[HۘHXܘYY[\[ۋY[[[ M [\[[ۜ\H[\Z][H\YY]HH\\Y[و[\ۛY[[X[ۈ T H[\H[][]Y\[ܘ[[\X]]Y\[ PH[\\H[[Y[XX]\\[\[]]H[ˈ]\H[][]Y\™^\Y\[\ۘ\X]Z\^\HH[YX][\و[\[[ۜˈ\ۙ\Hۘ\BXXX[[[\ۛY[[Xܘ]ܚY\ S K]\^B\\Y[وX[  K\\Y][\ۛY[[[\][ۘ[X[[Y[\[]]H SJHH]\•[]\]H[]\^H\\Y[و[\ۛY[[X[ۂT K[]X]Y[^\HYH[ۚ]܈H[HقH[\[Hو[\Y[Y\[\[[ۜˈ\œYK\[H۝[Y[H[\[\X Z[\YX\\BH[Y][ۘ[ LHT \Y[Y] B[\[H܈YHۜX]]HYX\ۘH\YX\܈H[ق[[Y\ NPPUTB[Y][ۈوHYY]Y]܈YX\\[‘^\H\[ܛ[[X[\ TH[B]]YH[[ۚ]ܚ[YB\[Hۘ[][ۈوXܚ[]Y\[[ МB[]\^H\Y[