Lab Matters Summer 2018 - Page 50

APHL 2018 Annual Meeting Poster Abstracts Rapid Concentration of Biological Particles from Environmental Samples A. Lewis, M. Hornback and A. Page, InnovaPrep, Drexel, MO InnovaPrep has developed a suite of systems for concentration of bacteria and other biological particles from liquid samples. Volumes of water from a few milliliters to tens of liters of water are processed through flat or hollow fiber membrane filters to capture any biological particles that are present. The biological particles are then efficiently recovered from the membrane surface with a tangential flush using carbonated “wet foam.” The wet foam is expanded up to six times the o riginal liquid volume and becomes highly viscous, allowing it to act at the membrane surface and recover the particles into volumes significantly smaller than can be attained with traditional liquid elutions. The process is scalable, efficient and typically results in concentration factors of approximately 1000X per concentration stage. The CP Select, launched in September 2017, is the second generation of InnovaPrep’s Concentrating Pipette (CP) instrument. The CP Select uses Concentrating Pipette Tips (CPT) to aspirate and concentrate bacteria from a large input sample and then dispense a final concentrated sample. CPTs are disposed of after each sample, enabling the CP to be utilized for concentration of pathogens and indicator organisms from a variety of aqueous samples without cross contamination. Two types of flat membrane filter CPTs and four types of hollow fiber membrane filter CPTs, allow the system to be used for a variety of sample types and a variety of organisms, including viruses, bacteria and parasites. Concentration of 100 mL water sample and 1 L water sample, experimentally spiked with 100 CFU Escherichia coli were performed using InnovaPrep’s CP Select liquid concentrator using 0.2 µm hollow fiber Concentrating Pipette Tips (hf-CPTs) and 0.075% Tween 20/PBS elution fluid. Concentration of 100 ml spiked PBS resulted in an average recovery efficiency of 78.8% in the first elution, with an average elution volume of 337 µL and an average processing time taking 0.8 minutes. Recovery efficiencies for 1 L spiked PBS were similar to data from 100 mL runs, with 75.3% recovery in an average of 362 µL elution volume. Overall recovery increased to 85.19% when combined with a second elution. Overall time to concentrate 1 L of spiked fluid took 7.22 minutes. Presenter: Ariel Lewis, InnovaPrep, Drexel, MO, Phone: 816.619.3375, x104, Email: alewis@innovaprep.com of preparedness measures for healthcare partner response to public health emergencies, such as chemical threats. Robust Level 3 programs, developed by state member laboratories, include healthcare partner training, exercises and outreach. Whether a state, territorial or metropolitan area, each LRN-C laboratory is unique with regard to demographics, size and hospital system. To aid Level 3 Coordinators to fill any gaps in their programs, a resource guidance document was proposed by CT-CWG members. This document would include practical information that could be used to develop a robust Level 3 program with consideration for each jurisdiction’s specific needs. APHLs Environmental Health Committee (EHC) and eleven volunteers from the CT-CWG provided subject matter expertise for development of a universal guidance document for the Level 3 Coordinators. Meetings hosted by APHL members were held utilizing an APHL SharePoint site for efficient coordination of conference calls and email. The group established experience based recommendations that would be most helpful for Level 3 activities. The group also solicited ideas from the entire APHL CT-CWG. The workgroup met frequently to discuss progress and suggest edits for improvements to the Level 3 Resource Handbook. Since the first version release, minor revisions were made to the document in March 2015. December 2016, the EHC proposed a refresh of the Level 3 Resource Handbook. Short term goals included addition of an acronym list, repairs to URLs and Emergency Response Laboratory Network (ERLN) information. Long term goals were identified for the training section of the guidebook that would include enhancement of training program practices and an exercise experience library as portrayed by the different member states. Short term revisions were completed in September 2017 by a smaller group of CT-CWG members using similar communication paths developed during the making of initial version. Our poster will announce and share the remake of the (LRN-C) Level 3 Resource Handbook. Presenter: Teresa Miller, Michigan Department of Health and Human Services, Lansing, MI, Phone: 517.241.0925, Email: millert28@michigan.gov Detection and Characterization of Salmonella in Environmental Samples (complete abstract in Food Safety, p. 57) Triple Quadrupole ICP-MS for Biomonitoring Applications Laboratory Response Network — Chemical (LRN-C) Level 3 Resource Handbook K. Aviado, C. Dingman, J. Schneider and C. Bean, New Hampshire Public Health Laboratories, Concord, NH S. Yerabati 1 , T. Miller 2 , R.E. Nickla 3 ; 1 California Department of Public Health, Richmond, CA, 2 Michigan Department of Health and Human Services, Lansing, MI, 3 Oregon State Public Health Laboratory, Hillsboro, OR Triple quadrupole inductively coupled plasma mass spectrometry (ICP-QqQ-MS) offers many advantages over traditional single quadrupole (ICP-Q-MS), including enhanced analyte selectivity, sensitivity and matrix tolerance, making it a useful tool for performing highly sensitive trace elemental measurements in a variety of clinical matrices for biomonitoring public health studies. The Biomonitoring Program at the New Hampshire Public Health Laboratories (NH PHL) employs triple quadrupole ICP-MS to assess exposures to trace metals in communities around the state. The NH PHL has developed several analytical methods on a triple quadrupole (ICP-MS) to conduct two ongoing public health studies: a targeted investigation that assesses exposure to arsenic and uranium from private well water in high-risk communities around the state and a surveillance study that monitors for toxic metals The APHL Chemical Threat Collaborative Workgroup (CT-CWG) developed the Laboratory Response Network-Chemical (LRN-C) Level 3 Resource Handbook. The guide was designed to be used by LRN-C member partners and coordinators who provide Level 3 outreach to healthcare facilities, first responders and any other agency expected to respond to a chemical release resulting in human exposure. The first version was released in November 2014 and was placed on the LRN-C Toolkit APHL SharePoint site. Specific funded benchmark Level 3 capabilities, as defined by the Centers for Disease Control and P revention (CDC), should be sustained, including the provision 48 LAB MATTERS Summer 2018 PublicHealthLabs @APHL APHL.org