Hooo-Hooo Volume 11 Nr 2 - Page 7

• • • Blood smear • • Blood smear explanation 2 smears. Let the smears dry, protecting them from flies which will eat the blood off the slide. Label one end with a pencil (date, species, animal ID) and wrap them in paper with cellotape to prevent them from breaking. One smear should be fixed in 70-100% ethanol for cytology (with the pathology samples); the other should be air dried and given to Prof. Jansen. • • • • For pathology (Research and Scientific Services, National Zoological Gardens of South Africa). If at any point during the post mortem, any obvious abnormalities are seen, take photographs of the abnormality. Complete the specimen information sheet, listing all relevant history, and a description of any abnormalities you have seen. Use the following terms in your description: location, number and distribution, color, size, shape, consistency and texture. For example: “The liver contains multiple tan, firm nodules ranging from 1 to 3 cm in diameter that are distributed throughout all liver lobes. The nodules are gritty on cut surface.” Sample these areas, making sure to sample at the margin of the abnormality - include the abnormal region and the adjacent normal tissue so that your pathologist can orient the lesion. Remember to handle tissues very gently, holding them at the edge, and cutting them with a sharp knife. Make impression smears of organs by lightly touching a glass slide to the surface (air dried) Place tissues in formalin immediately. All tissues for formalin-fixation should be no more than 1 cm thick in any dimension and tissue:formalin ratio should be 1:10. Never freeze formalin fixed tissues. All tissues collected can go in the same formalin bottle. Use buffered formalin wherever possible. Formalin-fixed samples can be kept at cool room temperature until shipped. Ribs, liver, kidney spleen should be frozen for microbial, nutritional and toxicology studies. For DNA (Research and Scientific Services, National Zoological Gardens of South Africa): • • Small samples (3-5mm dia) of blood and tissues can be frozen in sterile containers Blood can be air dried on blotting paper provided for sampling, or preserved in absolute ethanol or a salt saturated DMSO solution. For Dr A Bastos (University of Pretoria): • Small sections of heart, liver, lung, spleen, muscle, kidney and pancreas – 100% ethanol in Eppendorf tubes. For Professor Jansen (TUT): • The entire reproductive tracts (male and female) – representative samples of the various portions to be forwarded to Dr Lane for histology when the reproductive tract has been studied. • The entire gastro-intestinal tract - representative samples of the various portions to be forwarded to Dr Lane for histology when the digestive tract has been studied. NECROPSY PROCEDURE Place the pangolin on its back. • Examine the skin for wounds and note the condition of the hair coat. 2017 September 7