Hooo-Hooo Volume 11 Nr 2 - Page 5

Field Necropsies of Pangolin A Manual for Compiled with the Tshwane University of Technology and Pangolin Working Group Free ranging animals usually die naturally, are hunted or culled in the field, far from laboratory or pathology services. The information each animal can yield is critical in assuring early diagnosis of the cause of its death, prevention of spread of infectious disease to other animals and people, and in providing general baseline information on the anatomy, histology, haematology, metabolic profiles and patterns of disease in different species. Samples can be stored and made available for future research worldwide. Necropsy procedure is standard for all mammals, apart from some differences in how to sample the gastrointestinal tract. Detailed knowledge of anatomy is not necessary to collect and store sets of tissues and organs, which may yield valuable information. We here describe a rapid procedure designed for arduous conditions and speed.This is a simple field necropsy technique, with suggested sampling protocols, designed to maximize the value of information that can be obtained from each unfortunate dead animal. PLEASE USE THESE REFERENCES: 1. African Pangolin Working Group. Standardized data collection protocol for Ground Pangolins (Smutsia temminckii) www.pangolin.org.za 2. African Pangolin Working Group. Ground Pangolin (Smutsia temminckii) DNA sampling protocol. www.pangolin.org.za 3. Munson, L. Necropsy of Wild Animals. Wildlife Health Center, School of Veterinary Medicine, University of California, Davis 4. Murnane R, Kinsel M J 2000 Mammal field necropsy protocol. University of Illinois Zoological Pathology Program 5. Woodford M H, Keet D F, Bengis R G 2000 Post- mortem procedures for wildlife veterinarians and field biologists. Office International des Epizooties, Care for the Wild and the Veterinary Specialist Group/Species Survival Commission of the World Conservation Union (IUCN) 6. OIE 2010 Training Manual on Wildlife Diseases and Surveillance. Office International des Epizooties website www.oie.int BEFORE YOU LEAVE FOR THE FIELD Check you have the tools you will need: • Essential items: sharp knife and scissors; sample bottles, 10% buffered formalin, 10% ethanol and ziploc bags; disposable apron, gloves, boots and mask; paper towels, string, cellotape; 2017 September 5