Hooo-Hooo Hooo-Hooo Volume 12 Issue 01 | Page 13

kg). The inductions were calm and smooth and mean induction time was 4.0±1.1 minutes. Heart rate (mean: 50±9 beats minute-1) and respiratory frequency (mean: 20±3 breaths minute-1) were stable throughout immobilization. Hypertension (mean arterial blood pressure: 167±19 mmHg) was noted in most of the animals - this has been reported by a number of authors in cheetah immobilized with different drug protocols and may be a physiological characteristic of this species. The mean recovery time after reversing with naltrexone and atipamezole was 9.1±3.6 minutes. Giraffe: The Bamanil volume dose rate ranged from 4-6 ml per animal for sub-adult giraffes. The mean induction time to recumbency was 12 minutes. No apnoea was observed in any of the giraffe. The mean heart rate was 37±14.3 beats minute-1 and mean respiratory frequency was 64±10.4 breaths minute-1. Blood pressure increased over time (mean arterial pressure: 143±43.5 mmHg). Time to full recovery was 5 minutes from injection of reversals. No adverse effects or re-narcotizations were observed after recovery. No mortalities during anesthesia were observed in this study. Giraffes were intubated, head elevated and the neck received massage during immobilization in this study. In trained African elephants: The body weight of the animals was estimated based on shoulder height and ranged from 1100-4000kg. The Bamanil volume dose rate used for the standing sedation of 14 African elephants ranged from 0.0006-0.0008 ml/k (mean: 0.0007 ml/kg). First signs of sedation were noted at 6.1±2.9 minutes after darting. Signs of sedation were observed as drooping ears, relaxation of the trunk with the tip resting on the ground and prolapse of the penis in bulls. Animals were approached after 17-20 minutes after darting. The mean overall heart rate was 39.6±6.5 beats minute-1, respiratory frequency was 6.1±1.7 breaths minute-1 and mean arterial pressure was 98.3±16.2 mmHg. Time to first signs of recovery was observed at 2.5±1 minutes after reversal. Animals didn’t react to painful stimuli such as micro- chipping, wound treatment and vaccination. Conclusion The Bamanil combination proved to be a reliable and reversible veterinary anaesthetic for the immobilization of lions, cheetahs, blesbok, giraffes and for the standing sedation of trained African elephants. Acknowledgements We would like to give special thanks to Dr Peter Rogers, Mrs Adine Roode, the Hoedspruit Endangered Species Centre (HESC), Camp Jabulani and Moholoholo Rehabilitation Centre as well as all the staff at these facilities. Without your assistance, effort and support, this study would not have been possible. 1Institute of Veterinary Medicine and Animal Sci- ences, tonian University of Life Sciences, Estonia 2Wildlifevets.com, Ngongoni, Mpumalanga, South Africa 3Wildlife Pharmaceuticals South Africa (Pty) Ltd., White River, South Africa 4 Department of Animal Science, Univer- sity of Stellenbosch, South Africa 2018 MAY 13