HHE Pathology and diagnostics supplement 2018 - Page 11

the ClearCell FX technology uses Dean Flow and hydrodynamic focusing within a curved channel for differential collection of CTCs and WBC streams. 25 However, this method includes lysing the RBCs prior to isolation of CTCs. Such technologies or approaches have the advantages of being less complicated, sometimes rapid, and requiring minimal equipment. However, some of these approaches may be prone to clogging and the release of the CTCs into suspension for further analysis is challenging. a substantial number of leucocytes, and CTCs need to be identified specifically at the single-cell level by a robust and reproducible method that can distinguish them from normal blood cells. Immunological techniques CTCs can be detected by using a combination of membrane and/or intra-cytoplasmic anti- epithelial, anti-mesenchymal, anti-tissue-specific marker or anti-tumour-associated antibodies. 11 However, many CTC assays use the same identification step as the CellSearch ® system: cells are fluorescently stained for cytokeratins (CK), the common leucocyte antigen CD45 and a nuclear dye (DAPI). Through multicolour image analysis with a fluorescence microscope, CTCs are defined as 44CCR("D6V2FVv6RFvV0&RƖ6&RFf&W2FffW&VB66W"GW0f"WR4f"'&V7B6B&7FFP66W"BFW"WFVƖGVW'2F77VRЧ7V6f2FvV2&R67VF&R4f &7FFR66W"VG&ֆv7VVBWFFVBFvF֖7&66W6rf&W"F2'&66rFV6wd5B2&VVFWfVVBFFWFV7B5D72F@&R&VVB'F&FW2vFfV&W66V@6VvFW233"FW"6ƖFR&6VBWFFV@66r֖7&66W2fR&VVG&GV6V@f"FWFV7Fr5D726VFrFR66R *খvr77FVBFR& *77FV323B@6r&֗6r&W7VG2FB7FVVBF&PfƖFFVB&vR6Ɩ67GVFW26P&W6V&6W'26W6Rfr7FWG'FV6wv6ffW'2vF&VvWBFWFV7F@&VƖrb6WfW&F&vWG2BFR6RFR "F&vWG23R&VfW"6R7FWFW'2r6V6'Frf"7B&6W76rǗ623bFVVBVv6FWFV7FffW'2FRGfFvR`vr6Fb7FVB5D72f"7V'6WVV@V7V"6&7FW&6FvRV6F'֖7&VFb5D72076&R3rB2&FW"&GVW2BFRЦ67V֖sFW&FfRWFFVB6vR6V6VV7FFWf6R2FW&Vf&R&VVFWfVVBFRDU'&(J"FV6w&6VBFVV7G&&W627G&FVw'G&r6vP6V2DU6vW232FW6vVBf"6vR6V&V6fW'b5D72VFR6Ɩ67GVFW2fPW6VBDU'&(J"FFWFV7BB&V6fW"6vP5D72f"7V'6WVVBvVWF2Ǘ6W2fvpV&6VB3( 3CFRW6RbF2FV6wf 5D2&V6fW'vƖVǒ&RƖ֗FVBF6W0vF&VFfVǒvV&W"b5D72GVRF6V72b&FVǒCRGW&r6PFrC FVV7G&&W626VV7FfFfR&6f"6V6W&FFVV7G&&W62DUWG2FRF7F7@VV7G&6fvW'&G2bFffW&VB6V2v6FWVBFV"66Ff"WR6VV'&RV6WW2&vVW2'wf"WR6R6RBVGR7G&V *6W&677FVf"5D0V&6VBƖW2FRf'7B7G&FVwF&VvFRW6Rb֖7&fVF2FWf6RW6rDUfVBfr767B6V6W&FFV6VR#`6W7FW"6VV7FfWrWFG2fR&VVFWfVVBf"7V6Ɨ6V@FWFV7Fb5D26W7FW'27B66W25D06W7FW'2vW&R'6W'fVBvVFWFV7FrFfGV5D72VFR7GVFW2W6r֖7&fG&Ff 5D26FfR&W'FVB6GW&r5D06W7FW'2֖7&V&ƒ#~( 3#FW6R&W'G0F6FRFB5D26W7FW'27FF7BvVগ6FVBvF֖7&fG&FWFG2vW&V0FW"V&6VB7G&FVvW2֖vBVFW"fF6GW&R6W7FW'2"'&VFV'B6&vPWB&W'FVBFRFWfVVBbfVF&VRFV6֖7&fG&F77FVFP6W7FW"67V6f6ǒFW6vVBF6GW&R5D06W7FW'23FR6R'WB67F6FVBFW6vbFR6W7FW"66GW&W2FW6R6&7VFpGVW"֖7&V&ƒW6rVFR&w2`6gFVBG&wV"'27G&FVvW2f"5D2FWFV7FgFW"V&6VBFR5D2g&7F7F6F0F&R7G&FVvW2f"6&7VFrGVW"6VV&6VBBFWFV7FV&6V@&v6&W'FW0( 26FfR6VV7F6V6V&66fWw7vW FFW7@WW65D2650( 2VvFfR6VV7F&6WGFU6W50V76W5D2֔6FWFV7F( "Vv6( "V7V ( "gV7F( 2ffGVW"Vw&( 2fG&U5@f676V6V26B&6VB7G&FVvW0V6V26B&6VB5D2FWFV7FWFG2&RFP7BvFVǒW6VBFW&FfW2FVv6762FW6RFV6vW2FVFg7V6f0GVW"D"$F6f&FR&W6V6Pb5D72F&V7FǒC2FWFV7FffW2FW6vp7V6f2&W'27W6VFǒ766FVBvF5D27V6f2vVW2FW6RvVW2VFW"6FRf F77VR&v"GVW"7V6f2&FV2"&R7V6f6ǒ6FvWFF2G&66F2"WFFGFW&2fVB66W"6V2C@FRV6V26B&6VBWFB2FRvW7@6V6FfG'WB627V6f6GvrFFPFVFb6GW&VB66W&W26V2F66&W'FW0( 2FV6G6VV7Ff6VP6V6( 2FVV7G&&W626VV7F7G&VЮ( 26R6VV7F67&VV6V4U@'6'F6V6WfPR#7FƆVF6&R6