Functionele stabiliteit van Biopolymeren | Page 140
KORTE INLEIDING VAN HET ONDERZOEK
This project is part of the research that is done by Waterschap Brabantse Delta to
create valuable products from process waters discharging on one of their waste water
treatment plants using the activated sludge from this plant.
DOEL VAN HET ONDERZOEK
To determine the possibilities to produce PHA (by bacteria) from COD-rich (COD =
chemical oxygen demand) process water from companies in the neighbourhood of
the treatment plant Bath with the bacteria in the activated sludge of this plant. Also
literature research has been done to determine the optimal conditions to produce
PHA.
AANPAK
To achieve this goal, there are four main steps.
1.
First a literature review to determine the optimal condition for PHA
accumulation.
2.
Next the selection of suitable process water streams takes place based on the
content of these streams. Favourable conditions are a high COD-concentration
and low N- and P- concentrations
3.
As a third step the companies are contacted and asked for their cooperation.
Process water samples were collected from the cooperating companies.
4.
Finally the collected process water samples were tested to determine the
possibilities of PHA-production.
RESULTATEN
In total 24 companies (out of more than 100 companies) have a process water stream
that might be suitable for PHA production. These companies were contacted. 15
companies were willing to cooperate. From 10 companies a sample of their process
water stream was collected. The other 5 companies did not have time during the
timespan of this project.
For each of the 10 process water streams it was tested (in batch experiments) if PHA
production with activated sludge from wastewater treatment plant Bath was possible.
With 7 process water streams the single cell bacteria accumulated up to 80% of their
cell area with PHA (shown by colouring with Nile red). With the other process water
streams PHA accumulation was much lower (up to 40%). The accumulation of PHA in
strands of bacteria, which were also present in the samples, was considerably lower (up
to 20%). Maybe because these are different type of bacteria.
It can be concluded that PHA accumulation on COD-rich process water streams
is possible. PHA accumulation up to 80% of the area of single cell bacteria occurs.
Further research should focus on the characterization of the process water streams and
the type and amount of PHA that can be formed.
BEDRIJFSGEGEVENS / CONTACTGEGEVENS:
Naam:
Waterschap Brabantse Delta
Contactpersoon:
Etteke Wypkema
E-mail:
[email protected]
Namens de opdrachtgever heeft Etteke Wypkema voor vrijgave getekend en heeft van onderzoeker
Jack van Schijndel (www.biopolymerengroep.nl) hier ook een exemplaar van ontvangen.
140