Forensics Journal - Stevenson University 2014 | Page 7

STEVENSON UNIVERSITY Due to this mandate, the FBI was able to access the database that held every transfer of Anthrax across the country to determine which laboratories had access to the Anthrax strain in question. From this database, a list of institutions with Anthrax in their possession was generated. This reduced the potential laboratory sources to 16 located in the United States and 4 located internationally that possessed the Ames strain of Anthrax. Obtaining the samples from laboratories located within the United States was relatively easy since we were able to use the court system to subpoena the laboratories. However obtaining the samples from laboratories overseas was a more arduous task. We could not use the legal system and had to rely on diplomatic means, which took much longer. We eventually were able to collect samples from Canada, United Kingdom, and Sweden. Senator Leahy with identical handwriting, return address, and postage as the letter sent to Senator Daschle. This unopened letter contained nearly 1 gram of Anthrax powder which allowed us the freedom to conduct testing that previously was not available because of the small samples we recovered from the opened letters. It opened the door for Carbon-14 dating, which was very helpful in determining the age of the Anthrax spores. In this case, two laboratories were able to conduct Carbon-14 dating and they both came to the same conclusion: the Anthrax spores were modern and dated from 1998-2001. This was especially important because the government had forbidden the use of Anthrax as a weapon in 1969, at which point, there were hundreds of pounds of classified dried Anthrax powder in the country’s arsenal. The Carbon dating analysis showed that the samples used in this attack did not come from the government supply. Once we obtained these Ames samples, we compared them against the Ames that was found in the mailed letters. We used DNA fingerprinting to identify a unique genetic signature in the mailed spores. From this information, we were able to determine that the attack Ames originated from the United States Army Medical Research Institute of Infectious Diseases located in Frederick, Maryland (USAMRIID). HOW DID THE CASE RESOLVE? The samples were all shown to have originated from USAMRIID and were traced back to one scientist located there who was an Anthrax vaccine expert. Nine of the samples came from one flask which originally contained 1000 ml of Ames strain spores. The scientist named Bruce Ivins had contracted production of the spores for use in vaccine challenge studies, for which he was responsible in his duties at USAMRIID. The samples from the flask matched genetically with the samples taken from the mailed letters. At this time, an investigation ensued which included investigating his emails, computers, purchase history which showed some very incriminating evidence. Once it became clear that he was close to being indicted on terrorism charges that might include the death sentence, he committed suicide in July 2008. WHAT WOULD YOU SAY WAS THE BIGGEST BREAKTHROUGH IN THE CASE? There were two important breakthroughs during this case. First and foremost, the genetics used in this case was groundbreaking. Using newly available procedures in the field of microbiology, we were able to find unique mutations in ten of the 1000 plus samples we had collected. We were able to match the unique mutations in the samples collected from the letters with the samples in our collections of known exemplars of Anthrax. All ten matches came from the same location. This was the first breakthrough, which determined that the Anthrax came from USAMRIID. IN THE TERMS OF BIOLOGICAL TERRORISM ATTACKS, HOW WERE THE INVESTIGATORY TECHNIQUES USED IN THE ANTHRAX ATTACKS DIFFERENT FROM THOSE USED IN THE PAST? The second important breakthrough was finding the Senator Patrick Leahy letter in a batch of quarantined mail. After the letter sent to Senator Thomas Daschle was found on Capitol Hill, the Sargent in Arms of the House of Re