EVERSIGHT SERVICES
DMEK: DESCEMET MEMBRANE ENDOTHELIAL KERATOPLASTY
DMEK PREPARED TISSUE
Eversight Preparation Procedure
Tissue is mounted on a vacuum block to
perform a 360o partial trephination through
the Descemet membrane. Surgical grade
trypan blue is then used to stain the tissue for
increased visualization.
A 360o partial dissection is then performed
1-2mm interior to the trephination score.
Next, the Descemet membrane is manually
peeled from the stromal bed, leaving a small
hinge attached within the trephinated button.
After that, the Descemet membrane is gently
adhered onto the stromal bed, and the tissue is
placed in a corneal viewing chamber containing
Optisol GS.
Before transport to the surgery location,
the tissue undergoes additional specular
and slit lamp evaluations.
As with any new surgical procedure, Eversight
understands the importance of providing
flexible options to surgeons as techniques are
refined. If you have questions or need alternate
preparation options, please contact Eversight.
DMEK tissue specification options
Button Trephination
Single partial trephination: 9.5mm is the
standard and largest size offered.
Other options: 8.0mm, 8.5mm
and 9.0mm.
Orientation Markings
(Available by surgeon request)
Micro Stromal Stamp/
2mm Punch Combination
Before the Descemet membrane is returned
to the stromal bed, a 2mm hole is punched
through the stroma near the periphery of
the trephinated button. After the Descemet
membrane is returned to the stroma, the
tissue is placed epithelial side up and the
micro “S” is stamped onto the stroma through
the trephinated hole. The 2mm punch is
then replaced into the stromal bed.
Fold-Over Micro Stromal Stamp
Before the Descemet membrane is returned
to the stromal bed, the peripheral edge is
folded over onto the rim and the micro “S”
stamp is applied to the stromal side of the
Descemet membrane.
Eversight Support
Eversight is committed to ensuring that you are
both comfortable and confident as you begin
to offer DMEK to your patients. We provide a
number of practice opportunities, including wet
lab courses, training tissue, supply kits and use
of our facility with the assistance of an Eversight
processing technician.
See opposite side for diagrams.
Double partial trephination: 7.25mm, 7.5mm,
7.75mm, and 8.0mm.
Hinge Location
Our standard DMEK tissue has a peripheral
hinge located 1-2mm interior to the trephination
line, identified by a scleral marking.
Alternatively, a centrally placed 2x2mm
hinge is also available by request.
Questions? Contact: Lauren Johnson • [email protected] • (800) 247-7250 ext. 2597