Equine Health Update EHU Vol 20 Issue 02 - Page 22

EQUINE | Abstracts Association of Neutrophil Morphology with Bacterial Isolates in Equine Tracheal Wash Samples N. A. Jocelyn C. E. Wylie M. Lean A. Barrelet A. K. Foote Background Tracheal wash sample neutrophilia is common in lower airway inflammation of various causes; however, relevance of cytomorphological features to culture of bacterial pathogens has not been established. Objectives To investigate whether the presence of nondegenerate or degenerate neutrophils in tracheal washes is associated with culture of bacteria and, if so, whether this is influenced by age or temporal factors. Study design Cross‐sectional study. Methods Results Sufficient data for inclusion in the multivariable model for nondegenerate neutrophilia were available from 1100 horses. Culture of potentially pathogenic bac- teria was associated with increased odds of degener- ate neutrophilia compared with samples with nega- tive culture (OR 4.5, 95% CI 3.1, 6.4, P‐value<0.001). Horses over 9 years old had lower odds of having de- generate neutrophilia than those aged 1–3 years (OR 0.6, 95% CI 0.4, 0.9, P‐value<0.02). In the spring/ summer, horses had reduced odds of a degenerate neutrophilia compared with winter (OR 0.4, 95% CI 0.3, 0.7, P‐value <0.001). Main limitations Tracheal wash samples submitted to Rossdales LLP from 1/1/2013 to 31/7/2015 were evaluated using set criteria. Ne