Biotech 2nd Edition Sample Ch. 4 Biotechnology Sample Ch. 4 - Page 8

Lab: DNA Extraction (each lab group) 6. Transfer 10 mL of the 36-hour yeast broth culture into a 15-mL conical tube, and collect the yeast cells by centrifugation at 3000 rpm (high) for 10 minutes. 7. Discard the supernatant and resuspend the pellet of yeast cells in 0.5 mL of dH2O. 8. Add 1 mL of the Cell Lysis Solution and crush the cell mixture for 1 minute using a glass stirring rod. 9. Add 200 ┬ÁL of 5% protease and vortex the