Biotech 2nd Edition Sample Ch. 4 Biotechnology Sample Ch. 4 - Page 6

Data Analysis/Conclusion Discuss how easily the DNA could be pulled out of solution in long, spoolable strands. Did you and your partner have the same results as others? Why or why not? Discuss the value of learning how to separate pure DNA molecules from a known liquid solution. How would this technique be valuable to biotechnologists? Consider where DNA is found naturally. Thinking Like a Biotechnician 1. In this activity, you precipitated DNA, ordered from a biological supply house, out of a relatively pure solution. Which molecules did the supply company have to remove from the original cell source to purify this DNA? 2. If 100% of the DNA was recovered during spooling and transferred to the 2 mL of TE buffer in step 11, what would the approximate final concentration of DNA be in the tube? 3. Why is the final sample refrigerated? DNA Isolation and Analysis 71