Anuario Raza Polo Argentino Anuario2018 | Page 251

at any time, thereby permitting embryo production the year round. These embryos are frozen and transferred to receptor mares whenever the breeder wishes. Barren mares: once the immature oocytes are obtained through follicular aspiration, all the rest of the process occurs in vitro, which is ideal in the case of barren mares. Barren stallions: Since the spermatozoon is injected into the oocyte, we are in a way making things easier and it is not necessary that there be millions of normal sperms available. Limited reserve of frozen or low-quality semen: the use of frozen semen may be maximized since a semen straw may be cut into little bits or even in some cases allowed to thaw and then freeze again in more diluted form so as to be able to count on an almost unlimited reserve of semen. Dead mares: for cases in which a mare must be put down due to injury or sudden death, for example, in the case of a colic, it is possible to obtain a last pregnancy by taking oocytes to be found in the ovary. Immediately after the death of the animal, the ovaries must be sent to the laboratory where the oocytes are removed and cultivated in order to be later injected with a spermatozoon from the stallion chosen. This could have been the case of many famous mares that became injured while playing and were put down. Dead stallions: something similar could be done with stallions that for different reasons (colic; injury; accidents) die abruptly or must be put down. If the testicles are sent immediately to the laboratory, it is possible to obtain the sperm present in its epididymis and vas deferens. Those sperm cells are frozen and are enough to achieve pregnancies through ICSI technique. In summary: technological advancement in latter years has provided the breeder with new tools that make possible the production of female embryos at any time of the year, from both barren and fertile animals and which may be preserved frozen and transferred in order to schedule foaling. 249