Agri Kultuur November 2018 | Page 16

Researchers from the Prefectural University of Kumamoto in Japan conducted DO control experiments in a fish farm on red seabream with the microbubble generating system in Kusuura Bay, Amakusa, Kumamoto Prefecture, Kyushu, western Japan, between 2003 and 2007. The water depth of the bay was between 16 and 20 m at the centre of the bay. The annual temperature of the surface water ranged from 19.7°C to 29.3°C between May and October 2007. This study was financially supported by a Research and Development Program for New Bio-industry Initiatives of the Bio-oriented Technology Research Advancement Institution of Japan. The results of the experiments indicate that the control of DO levels of water in the net pen using the microbubble generator improved the growth rate of the fish significantly. In the control net pen, the body weight of the fish was 1.25 ± 0.17 kg in wet weight (mean ± SD, n = 30) at the beginning of the experiment on June 18, 2007, and they gained 1.39 ± 0.25 kg and 1.70 ± 0.26 kg by August 8 (50 days later) and October 3, 2007 (108 days AgriKultuur |AgriCulture later), respectively. In the experimental net pen, the body weight of the fish was 1.19 ± 0.24 kg in wet weight (mean ± SD, n = 30), slightly lighter than that of the control net pen at the beginning of the experiment. The difference in the mean body weight between the experimental net pen and the control net pen was not statistically significant (p = 0.15, Mann–Whitney’s U test, n = 30). The fish grew faster than that in the control net pen. The body weight reached 1.83 ± 0.21 kg in wet weight at the end of the experiment (108 days later), which was 0.13 kg heavier than that in the control net pen. The difference in body weight between these two net pens was statistically significant (p = 0.02, Mann– Whitney’s U test, n = 30). The increase in the body weight of the fish in the experimental net pen during the period of the experiment for 108 days was 0.64 kg in wet weight, which was 42% more than that in the control net pen (0.45 kg). In the experimental net pen, the body weight of the fish was 1.33 ± 0.22 kg in wet weight (mean ± SD, n = 30), slightly heavier than that 16